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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/15426

Title: Surface plasmon resonance-based DNA microarrays: Comparison of thiol and phosphorothioate modified oligonucleotides
Authors: JIMENEZ MONROY, Kathia
Kick, A.
EERSELS, Kasper
VAN GRINSVEN, Bart
WAGNER, Patrick
Mertig, M.
Issue Date: 2013
Publisher: WILEY-V C H VERLAG GMBH
Citation: PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 210 (5), p. 918-925
Abstract: We present the use of thiol-modified and phosphorothioate (PT)-modified oligonucleotides for building DNA microarrays on the gold surface of surface plasmon resonance (SPR) chips. PT-modified oligonucleotides (PTOs) have several advantages in comparison to thiol-modified ones. They do not form disulfides and the PT groups can be introduced in any desired position of the molecular backbone of the oligonucleotide. Additionally, modifications with PT groups are not as cost-intensive as thiol groups. The direct immobilization of the oligonucleotides via thiol and PT groups is compared. The affinity of the PT groups to gold is lower than that of thiol groups. Nevertheless, the hybridization kinetics of a model polymerase chain reaction (PCR) product could be studied in real time on a DNA microarray for both types of modified oligonucleotides. This contributes to clarification of previous, contradictory reports on the use of PTOs for their attachment to gold surfaces. The immobilization of PTOs could be improved by the introduction of iodoacetylated surfaces, which are reactive to bind PTs as well as thiols with high efficiency. Furthermore, the influence of the probe structure on the probe density and the hybridization was investigated.
Notes: Jimenez-Monroy, KL (reprint author), Tech Univ Dresden, D-01062 Dresden, Germany. Hasselt Univ, IMO IMOMEC, B-3590 Hasselt, Belgium. Kurt Schwabe Inst Mess & Sensortech eV, D-04736 Waldheim, Germany. kathia.jimenezmonroy@uhasselt.be
URI: http://hdl.handle.net/1942/15426
DOI: 10.1002/pssa.201200969
ISI #: 000319151900015
ISSN: 1862-6300
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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