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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/15421

Title: Electronic monitoring of chemical DNA denaturation on nanocrystalline diamond electrodes with different molarities and flow rates
Authors: Murib, M. S.
van Grinsven, B.
Grieten, L.
Janssens, S. D.
Vermeeren, V.
Eersels, K.
Broeders, J.
Ameloot, M.
Michiels, Luc
De Ceuninck, W.
Haenen, K.
Schoening, M. J.
Wagner, P.
Issue Date: 2013
Publisher: WILEY-V C H VERLAG GMBH
Citation: PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 210 (5), p. 911-917
Abstract: Probe DNA, consisting of a 36-mer fragment was covalently immobilized on nanocrystalline chemical vapour deposition (CVD) diamond electrodes and hybridized with a 29-mer target DNA. In this paper, we report on the label-free real-time electronic monitoring of DNA denaturation upon exposure to NaOH at different flow rates and molarities, using electrochemical impedance spectroscopy as readout technology. The impedance response was separated into a denaturation time constant and a medium exchange time constant by means of a double exponential fit. It was observed that the denaturation time is dependent on the flow rate as well as on the molarity of the NaOH. Surprisingly, it was observed that at low molarities (0.05M) the DNA does not fully denature at low flow rates. Only after flushing the flow cell a second time with 0.05M NaOH, complete denaturation was achieved. Confocal images were obtained and plotted in 3D graphs to confirm the results. This paper provides a systematic overview of measured denaturation times for different flow rates and at different molarities of NaOH. Optimization of these parameters can be a valuable asset in the field of mutation analysis.
Notes: Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium. Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany.
URI: http://hdl.handle.net/1942/15421
DOI: 10.1002/pssa.201200934
ISI #: 000319151900014
ISSN: 1862-6300
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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