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|Title: ||Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor|
|Authors: ||Huck, Christina|
Herrera, Cony N.
Baumann, Marcus E. M.
Schoening, Michael J.
|Issue Date: ||2013|
|Publisher: ||WILEY-V C H VERLAG GMBH|
|Citation: ||PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 210 (5), p. 926-931|
|Abstract: ||Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the welfare of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows MichaelisMenten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.|
|Notes: ||Aachen Univ Appl Sci, Inst Nano & Biotechnol INB, D-52428 Julich, Germany. Forschungszentrum Julich, Peter Grunberg Inst PGI 8, D-52525 Julich, Germany. Hasselt Univ, Inst Mat Res IMO, B-3590 Diepenbeek, Belgium.|
|ISI #: ||000319151900016|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2014|
|Appears in Collections: ||Research publications|
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