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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1535

Title: DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria
Authors: Geets, Joke
Borremans, Brigitte
Diels, Ludo
Springael, Dirk
Vangronsveld, Jaco
Van Der Lelie, Daniël
Vanbroekhoven, Karolien
Issue Date: 2006
Publisher: Elsevier
Abstract: A denaturing gradient gel electrophoresis (DGGE) method was developed to assess the diversity of dsrB (dissimilatory sulfite reductase β-subunit)-genes in sulfate-reducing communities. For this purpose a PCR primer pair was optimized for the amplification of a similar to 350 bp dsrB gene fragment that after DGGE gel electrophoresis enabled us to discriminate between dsrB genes of different SRB-subgroups,-genera and -species. The dsrB-DGGE method revealed considerable genetic diversity when applied to DNA extracts obtained from aquifer samples that were derived from monitoring wells of an in situ metal precipitation (ISMP) pilot project conducted at the site of a non-ferrous industry or from environmental heavy metal contaminated samples. The sequences of the excised and sequenced DGGE bands represented dsrB genes of different SRB-subgroups,-genera and -species, thus confirming the broad applicability of the PCR primer pair. Linking the results of the physico-chemical follow-up of the field and lab experiments to the dsrB-DGGE data will provide a better understanding of the contribution of the SRB populations to the ongoing ISMP processes.
URI: http://hdl.handle.net/1942/1535
DOI: 10.1016/j.mimet.2005.11.002
ISI #: 000238963000002
ISSN: 0167-7012
Category: A1
Type: Journal Contribution
Validation: ecoom, 2007
Appears in Collections: Research publications

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