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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/15163

Title: The high auto-aggregative and adhesive phenotype of the vaginal Lactobacillus plantarum strain CMPG5300 is sortase-dependent.
Authors: Malik, S
Petrova, MI
Claes, IJ
Verhoeven, TL
Busschaert, P.
Vaneechoutte, M
Lievens, B
Siezen, RJ
Balzarini, Jan
Vanderleyden, J
Lebeer, S
Issue Date: 2013
Abstract: Lactobacilli are important for the maintenance of a healthy ecosystem in the human vagina. Various mechanisms are postulated but so far are poorly substantiated by molecular studies, such as mutant analysis. Bacterial autoaggregation is an interesting phenomenon that can promote adhesion to host cells and displacement of pathogens. In this study, we report on the identification of a human vaginal isolate, Lactobacillus plantarum strain CMPG5300, which shows high autoaggregative and adhesive capacity. To investigate the importance of sortase-dependent proteins (SDPs) in these phenotypes, a gene deletion mutant was constructed for srtA, the gene encoding the housekeeping sortase that covalently anchors these SDPs to the cell surface. This mutant lost the capacity to autoaggregate, showed a decrease in adhesion to vaginal epithelial cells, and lost biofilm-forming capacity under the conditions tested. These results indicate that the housekeeping sortase SrtA of CMPG5300 is a key determinant of the peculiar surface properties of this vaginal Lactobacillus strain.
Notes: Reprint Address: Lebeer, S (reprint author) - Katholieke Univ Leuven, Ctr Microbial & Plant Genet, Louvain, Belgium. E-mail Addresses:sarah.lebeer@ua.ac.be
URI: http://hdl.handle.net/1942/15163
DOI: 10.1128/AEM.00926-13
ISI #: 000321255600008
ISSN: 0099-2240
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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