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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14991

Title: A Comparative Study of Soluble Protein Extractions of Populus deltoides × (Trichocarpa × Deltoides) for 2-DE
Authors: Dupae, Joke
Cuypers, Ann
Noben, Jean-Paul
Boulet, Jana
Weyens, Nele
Verstraelen, Karen
Vangronsveld, Jaco
Issue Date: 2012
Citation: Journal of Life Sciences, 6 (9), p. 970-979
Abstract: Background: The disclosure of the poplar genome strengthens its position as well-established model organism. Populus has been subject of several proteome studies, but up to date no comparative study was performed on the extraction method of soluble proteins for this species. The extraction is the most critical step in two-dimensional gel electrophoresis and each extraction method has its advantages, disadvantages and limitations. Therefore protein extraction methods should be optimized for each tissue before starting an experimental setup. In prospect of future DIGE (Differential Gel electrophoresis) experiments for the investigation of the effects of cadmium and inoculation with plant growth promoting bacteria at the proteome level, the aim of this study was to optimize an extraction method for soluble proteins of poplar leaves and roots. Results: The acetone-phenol extraction method was found to be the most suited, rendering a high spot number and low background interference. During further optimization, several critical steps in the extraction method were revealed. Conclusion: Aiming to optimize the extraction of soluble leaf and root proteins of Populus deltoides × (trichocarpa × deltoides) compatible with DIGE analysis, a protocol rendering high reproducibility, low background interference and a high spot number was established, however no novel insights were acquired.
URI: http://hdl.handle.net/1942/14991
Link to publication: https://www.academia.edu/26211541/Journal_of_Life_Sciences_Volume_6_Number_9_September_2012_Serial_Number_53_
ISSN: 1934-7391
Category: A2
Type: Journal Contribution
Appears in Collections: Research publications

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