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|Title: ||Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)|
|Authors: ||AMELOOT, Marcel|
Van de Ven, Martin
Ulises Acuna, A.
|Issue Date: ||2013|
|Publisher: ||INT UNION PURE APPLIED CHEMISTRY|
|Citation: ||PURE AND APPLIED CHEMISTRY, 85 (3), p. 589-608|
|Abstract: ||After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.|
|Notes: ||[Ameloot, Marcel; vandeVen, Martin] Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium. [Ulises Acuna, A.] CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain. [Valeur, Bernard] CNAM, Dept CASER, F-75141 Paris 03, France. [Valeur, Bernard] ENS Cachan, Lab PPSM, F-94235 Cachan, France.
|ISI #: ||000315186600009|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2014|
|Appears in Collections: ||Research publications|
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