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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14781

Title: A mutant of the Arabidopsis thaliana LIPOXYGENASE1 gene shows altered signalling and oxidative stress related responses after cadmium exposure
Authors: Keunen, Els
Remans, Tony
Opdenakker, Kelly
Jozefczak, Marijke
Gielen, Heidi
Guisez, Yves
Vangronsveld, Jaco
Cuypers, Ann
Issue Date: 2013
Publisher: ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Citation: PLANT PHYSIOLOGY AND BIOCHEMISTRY, 63, p. 272-280
Abstract: Lipoxygenases (LOXes, EC 1.13.11.12) are involved in growth, development and responses to stress. Earlier results suggested a role in stress generation, signalling and/or responses when Arabidopsis thaliana is exposed to cadmium (Cd), and expression of the cytosolic LOX1 was highly upregulated in the roots after Cd exposure. To investigate the involvement of LOX1 in early metal stress responses, three-week-old wild-type and lox1-1 mutant A. thaliana plants were acutely (24 h) exposed to realistic Cd concentrations (5 and 10 mu M) and several oxidative stress and signalling related parameters were studied at transcriptional and biochemical levels. Transcription of several genes encoding ROS producing and scavenging enzymes failed to be induced up to wild-type levels after Cd exposure. Expression of 9-LOX enzymes was inhibited in lox1-1 mutant roots due to lack of functional LOX1 and downregulated LOX5 expression, and the lox1-1 mutation also interfered with the expression of genes involved in jasmonate biosynthesis. LOX1 and RBOHD may be involved in stress signalling from roots to shoots, as the induction of APX2 expression, which is dependent on RBOHD activity, was disrupted in lox1-1 while RBOHD failed to be upregulated. A different pattern of H2O2 production and ascorbate and glutathione levels in lox1-1 mutants after Cd exposure may have indirectly influenced gene expression patterns. Although indirect effects of the lox1-1 mutation on gene expression complicate the determination of exact sensing - signalling - response pathways, the results presented here outline a more refined LOX1 functioning in Cd-induced stress responses that could be used in studies determining the exact involvement of LOX1 in these pathways. (c) 2012 Elsevier Masson SAS. All rights reserved.
Notes: [Keunen, Els; Remans, Tony; Opdenakker, Kelly; Jozefczak, Marijke; Gielen, Heidi; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Guisez, Yves] Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium.
URI: http://hdl.handle.net/1942/14781
DOI: 10.1016/j.plaphy.2012.12.005
ISI #: 000315011400034
ISSN: 0981-9428
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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