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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14705

Title: Probing live samples in second-harmonic generation microscopy using specific markers and fluorescent proteins
Authors: De Meulenaere, E.
Paesen, R.
Psilodimitrakopoulos, S.
Ameloot, M.
Loza-Alvarez, P.
Vanderleyden, J.
Issue Date: 2012
Citation: Periasamy, Amassi; König, Karsten; So, Peter T.C. (Ed.). Proceedings of SPIE (Multiphoton Microscopy in the Biomedical Sciences XII), (ART N° 82263C)
Series/Report: Proceedings of SPIE
Series/Report no.: 8226
Abstract: In an effort to complement cellular two-photon excited fluorescence (TPEF) microscopy with structural information from second-harmonic generation (SHG) imaging, we investigated the applicability of fluorescent proteins for SHG imaging. In the first stage, the first hyperpolarizability β, a measure for the second-order nonlinear optical properties of a molecule, was determined for several fluorescent proteins. In a second stage, an established HeLa cell line expressing a membrane protein labeled with a fluorescent protein, was adapted and imaged using simultaneous TPEF and SHG microscopy. The contour of stretched cells observed in these experiments was proven to be originating in microtubules instead of the fluorescent proteins.
URI: http://hdl.handle.net/1942/14705
DOI: 10.1117/12.907498
ISI #: 000302556900049
ISBN: 9780819488695
ISSN: 0277-786X
Category: C1
Type: Proceedings Paper
Validation: ecoom, 2014
Appears in Collections: Research publications

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