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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14469

Title: A role for TLR1, TLR2 and NOD2 in cytokine induction by Bacteroides fragilis
Authors: Stappers, Mark H. T.
Janssen, Nico A. F.
Oosting, Marije
Plantinga, Theo S.
Arvis, Pierre
Mouton, Johan W.
Joosten, Leo A. B.
Netea, Mihai G.
GYSSENS, Inge
Issue Date: 2012
Publisher: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation: CYTOKINE, 60 (3), p. 861-869
Abstract: Bacteroides fragilis, an intestinal flora commensal microorganism, is frequently isolated from abscesses and soft tissue infections. This study aimed to identify pattern recognition receptors (PRRs) involved in B. fragilis recognition and to characterize the induced cytokine profile. Human PBMCs were stimulated with heat-killed B. fragilis and cytokine levels were determined by ELISA. Roles of individual PRRs were assessed using specific blockers of receptor signaling pathways and PBMCs carrying single nucleotide polymorphisms of PRR genes. Cell lines expressing human TLR2 or TLR4 were employed to assess TLR-specificity of B. fragilis. TLR1, TLR2 and NOD2 were the main PRRs responsible for recognition of B. fragilis, while TLR4, TLR6, NOD1 and Dectin-1 were not involved. B. fragilis induced strong IL-6 and IL-8, moderate IL-1 beta and TNF-alpha, and poor IL-10, IL-17, IL-23 and IFN-gamma production. This study identifies the receptor pathways of the innate immune response to B. fragilis, and thus provides new insights in the host defense against B. fragilis. (C) 2012 Elsevier Ltd. All rights reserved.
Notes: [Stappers, Mark H. T.; Janssen, Nico A. F.; Oosting, Marije; Plantinga, Theo S.; Joosten, Leo A. B.; Netea, Mihai G.; Gyssens, Inge C.] Radboud Univ Nijmegen, Med Ctr, Dept Med, NL-6500 HB Nijmegen, Netherlands. [Stappers, Mark H. T.; Janssen, Nico A. F.; Oosting, Marije; Plantinga, Theo S.; Mouton, Johan W.; Joosten, Leo A. B.; Netea, Mihai G.; Gyssens, Inge C.] Radboud Univ Nijmegen, Med Ctr, N4i, NL-6500 HB Nijmegen, Netherlands. [Mouton, Johan W.] Radboud Univ Nijmegen, Med Ctr, Dept Med Microbiol, NL-6500 HB Nijmegen, Netherlands. [Stappers, Mark H. T.; Janssen, Nico A. F.; Mouton, Johan W.; Gyssens, Inge C.] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands. [Arvis, Pierre] Bayer Healthcare, Loos, France. [Gyssens, Inge C.] Hasselt Univ, Diepenbeek, Belgium. i.gyssens@aig.umcn.nl
URI: http://hdl.handle.net/1942/14469
DOI: 10.1016/j.cyto.2012.08.019
ISI #: 000311248000040
ISSN: 1043-4666
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Research publications

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