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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14367

Title: Hybridisation thermodynamic parameters allow accurate detection of point mutations with DNA microarrays
Authors: HOOYBERGHS, Jef
Carlon, Enrico
Issue Date: 2010
Citation: BIOSENSORS & BIOELECTRONICS, 26 (4), p. 1692-1695
Abstract: We consider mixtures of two DNA sequences t and t' differing by a single nucleotide, which are analyzed by an Agilent custom DNA microarray. In particular we focus on the case in which t, the "wild type", is predominantly abundant and t' the "mutant" is at very low concentrations compared to t. We show that by using appropriately designed arrays it is possible to accurately quantify the presence of t' even at low relative concentrations (approximate to 1%). The detection method is based on thermodynamic models of DNA hybridisation and on the analysis of a large number of hybridisation intensities from probes containing one or two mismatches with respect to t and t'. (C) 2010 Elsevier B.V. All rights reserved.
Notes: [Hooyberghs, Jef] Flemish Inst Technol Res VITO, B-2400 Mol, Belgium. [Hooyberghs, Jef] Hasselt Univ, B-3590 Diepenbeek, Belgium. [Hooyberghs, Jef; Carlon, Enrico] Katholieke Univ Leuven, Inst Theoret Phys, B-3001 Louvain, Belgium. jef.hooyberghs@vito.be
URI: http://hdl.handle.net/1942/14367
DOI: 10.1016/j.bios.2010.07.039
ISI #: 000286403400086
ISSN: 0956-5663
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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