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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14303

Title: MIP-based biomimetic sensor for the electronic detection of serotonin in human blood plasma
Authors: PEETERS, Marloes
Troost, F. J.
HOREMANS, Frederik
MURIB, Mohammed Sharif
Keszthelyi, D.
CLEIJ, Thomas
WAGNER, Patrick
Issue Date: 2012
Citation: SENSORS AND ACTUATORS B-CHEMICAL, 171, p. 602-610
Abstract: Serotonin is an important signaling molecule in the human body. The detection of serotonin is commonly performed by high performance liquid chromatography (HPLC), which is costly and time consuming due to extensive sample preparation. We will show that these problems can be overcome by using molecularly imprinted polymers (MIPs) as synthetic receptors in combination with impedance spectroscopy as readout technique. The MIPs were prepared with several blends of the underlying monomers and the best performing MIP material was selected by optical batch-rebinding experiments. MIP microparticles were then integrated in an impedimetric sensor cell and dose-response curves were measured in PBS buffer and in non-diluted blood plasma. The sensor provides reliable data in the physiologically relevant concentration regime as an independent validation by HPLC measurements demonstrates. Finally, we show that the impedimetric response upon serotonin binding can be attributed to a capacitive effect at the interface between the MIP particles and the plasma. (C) 2012 Elsevier B.V. All rights reserved.
Notes: [Peeters, M.; van Grinsven, B.; Horemans, F.; Alenus, J.; Murib, M. S.; Ethirajan, A.; Thoelen, R.; Cleij, T. J.; Wagner, P.] Hasselt Univ, Inst Mat Res IMO, B-3590 Diepenbeek, Belgium. [Troost, F. J.; Keszthelyi, D.] Maastricht Univ, Med Ctr, Div Gastroenterol Hepatol, Dept Internal Med, NL-6211 LK Maastricht, Netherlands. [Thoelen, R.] XIOS Univ Coll Limburg, B-3590 Diepenbeek, Belgium. [Wagner, P.] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium. marloes.peeters@uhasselt.be
URI: http://hdl.handle.net/1942/14303
DOI: 10.1016/j.snb.2012.05.040
ISI #: 000308572700077
ISSN: 0925-4005
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Research publications

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