Document Server@UHasselt >
Research publications >
Please use this identifier to cite or link to this item:
|Title: ||Changed gene expression in brains of mice exposed to traffic in a highway tunnel|
|Authors: ||Bos, Inge|
DE BOEVER, Patrick
Van Poppel, Martine
INT PANIS, Luc
|Issue Date: ||2012|
|Publisher: ||INFORMA HEALTHCARE|
|Citation: ||INHALATION TOXICOLOGY, 24 (10), p. 676-686|
|Abstract: ||Context: Air pollution has been suggested to have an impact on the brain. Objective: The objective was to assess the expression of inflammation-related genes in the brains of mice that had been exposed for 5 days to a well-characterized traffic-polluted environment, i.e. a highway tunnel. Materials and methods: Twenty C57BL6 mice were randomly allocated to four groups of five animals. Two groups were placed in the tunnel for 5 days (mean PM 2.5, 55.1 mu g/m(3), mean elemental carbon, EC 13.9 mu g/m(3)) in cages with or without filter, two control groups were housed outside the tunnel. Animals were assessed within 24 hours after the last exposure day. Lung injury and inflammation were assessed by bronchoalveolar lavage (BAL) and histology. Blood leukocytosis and coagulation parameters were determined in peripheral blood. The olfactory bulb and hippocampus were analyzed for changes in expression of inflammatory genes and brain-derived neurotrophic factor (BDNF). Results and discussion: Although carbon particles were abundant in alveolar macrophages of exposed mice and absent in non-exposed mice, there was no evidence of pulmonary or systemic inflammation. There was an increased expression of genes involved in inflammatory response (COX2, NOS2, NOS3, and NFE2L2) in the hippocampus of the exposed mice. In the olfactory bulb, a downregulation was found for IL1 alpha, COX2, NFE2L2, IL6, and BDNF. Conclusion: Although this short-term exposure to traffic-related pollution did not induce pulmonary or systemic inflammation, the expression of inflammatory genes was affected in different brain areas. The decreased BDNF expression in the olfactory bulb suggests lower brain neurotrophic support in response to traffic-related air pollution.|
|Notes: ||Panis, LI (reprint author),[Bos, Inge; Meeusen, Romain] Vrije Univ Brussel, Dept Human Physiol & Sports Med, Brussels, Belgium. [De Boever, Patrick; Nawrot, Tim] Hasselt Univ, Ctr Environm Studies CMK, Diepenbeek, Belgium. [Emmerechts, Jan] Univ Leuven, Ctr Mol & Vasc Biol, Louvain, Belgium. [Emmerechts, Jan; Vanoirbeek, Jeroen; Nemery, Benoit; Nawrot, Tim] Katholieke Univ Leuven, Unit Lung Toxicol, Dept Publ Hlth Occupat & Environm Med, Louvain, Belgium. [Panis, Luc Int] Hasselt Univ, Transportat Res Inst IMOB, Diepenbeek, Belgium. [Bos, Inge; De Boever, Patrick; Buekers, Jurgen; Van Poppel, Martine; Panis, Luc Int] Flemish Inst Technol Res VITO, Mol, Belgium.
|ISI #: ||000307903200007|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2013|
|Appears in Collections: ||Research publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.