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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/13709

Title: T4-Related Bacteriophage LIMEstone Isolates for the Control of Soft Rot on Potato Caused by 'Dickeya solani'
Authors: Adriaenssens, Evelien M.
Van Vaerenbergh, Johan
Vandenheuvel, Dieter
Dunon, Vincent
Ceyssens, Pieter-Jan
De Proft, Maurice
Kropinski, Andrew M.
NOBEN, Jean-Paul
Maes, Martine
Lavigne, Rob
Issue Date: 2012
Publisher: PUBLIC LIBRARY SCIENCE
Citation: PLOS ONE, 7 (3), p. e33227
Abstract: The bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage Vil. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and severity on potato tubers in laboratory assays. In addition, in a field trial of potato tubers, when infected with 'Dickeya solani', the experimental phage treatment resulted in a higher yield. These results form the basis for the development of a bacteriophage-based biocontrol of potato plants and tubers as an alternative for the use of antibiotics.
Notes: [Adriaenssens, Evelien M.; Vandenheuvel, Dieter; Dunon, Vincent; Ceyssens, Pieter-Jan; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, B-3001 Heverlee, Belgium. [Adriaenssens, Evelien M.; Van Vaerenbergh, Johan; Maes, Martine] Inst Agr & Fisheries Res, Unit Plant Crop Protect, Merelbeke, Belgium. [Adriaenssens, Evelien M.; De Proft, Maurice] Katholieke Univ Leuven, Div Crop Biotech, B-3001 Heverlee, Belgium. [Kropinski, Andrew M.] Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, Guelph, ON, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Transnat Univ Limburg, Diepenbeek, Belgium. rob.lavigne@biw.kuleuven.be
URI: http://hdl.handle.net/1942/13709
DOI: 10.1371/journal.pone.0033227
ISI #: 000303060800086
ISSN: 1932-6203
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Research publications

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