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Title: Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro
Authors: Boato, Francesco
Hechler, Daniel
Rosenberger, Karen
Luedecke, Doreen
Peters, Eva M.
Nitsch, Robert
Issue Date: 2011
Abstract: Pro-inflammatory cytokines such as interleukin-1 beta (IL-1 beta) are considered to exert detrimental effects during brain trauma and in neurodegenerative disorders. Consistently, it has been demonstrated that IL-1 beta suppresses neurotrophin-mediated neuronal cell survival rendering neurons vulnerable to degeneration. Since neurotrophins are also well known to strongly influence axonal plasticity, we investigated here whether IL-1 beta has a similar negative impact on neurite growth. We analyzed neurite density and length of organotypic brain and spinal cord slice cultures under the influence of the neurotrophins NGF, BDNF, NT-3 and NT-4. In brain slices, only NT-3 significantly promoted neurite density and length. Surprisingly, a similar increase of neurite growth was induced by IL-1 beta. Additionally, both factors increased the number of brain slices displaying maximal neurite growth. Furthermore, the co-administration of IL-1 beta and NT-3 significantly increased the number of brain slices displaying maximal neurite growth compared to single treatments. These data indicate that these two factors synergistically stimulate two distinct aspects of neurite outgrowth, namely neurite density and neurite length from acute organotypic brain slices.
Notes: [Boato, Francesco; Hendrix, Sven] Hasselt Univ, Dept Funct Morphol, Hasselt, Belgium. [Boato, Francesco; Hendrix, Sven] Hasselt Univ, BIOMED Inst, Hasselt, Belgium. [Hechler, Daniel; Rosenberger, Karen; Luedecke, Doreen] Charite Univ Med Berlin, Ctr Anat, Inst Cell Biol & Neurobiol, Berlin, Germany. [Peters, Eva M.] Charite, Charite Ctr Internal Med & Dermatol 12, D-10117 Berlin, Germany. [Peters, Eva M.] Univ Giessen, Dept Psychosomat Med, Giessen, Germany. [Nitsch, Robert] Johannes Gutenberg Univ Mainz, Univ Med Mainz, Inst Microscop Anat & Neurobiol, Mainz, Germany. sven.hendrix@uhasselt.be
URI: http://hdl.handle.net/1942/13500
DOI: 10.1186/1742-2094-8-183
ISI #: 000300342000001
ISSN: 1742-2094
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Research publications

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