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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/13370

Title: Heat-Transfer Resistance at Solid–Liquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA
Authors: van Grinsven, Bart
Vanden Bon, Natalie
Grieten, Lars
Murib, Mohammed
Jimenez Monroy, Kathia L.
Janssens, Stoffel D.
Haenen, Ken
Schöning, Michael J.
Vermeeren, Veronique
Ameloot, Marcel
Michiels, Luc
Thoelen, Ronald
De Ceuninck, Ward
Wagner, Patrick
Strauven, Hannelore
Issue Date: 2012
Publisher: American Chemical Society
Citation: ACS Nano, 6(3), p. 2712-2721
Abstract: In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.
URI: http://hdl.handle.net/1942/13370
DOI: 10.1021/nn300147e
ISI #: 000301945900089
ISSN: 1936-0851
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Research publications

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