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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/13172

Title: Real-time study of protein adsorption on thin nanocrystalline diamond
Authors: Grieten, Lars
Janssens, S.D.
Ethirajan, Anitha
Vanden Bon, Natalie
Ameloot, Marcel
Michiels, Luc
Haenen, Ken
Wagner, Patrick
Issue Date: 2011
Citation: PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 208(9), p. 2093-2098
Abstract: The study of protein adsorption on solid surfaces is interesting for theoretical and practical bio-analytical sensing applications. In this work we combine electrochemical impedance spectroscopy, enzyme linked immunosorbent assay, and fluorescence microscopy with thin boron doped nanocrystalline diamond films to address and study the adsorption behavior of globular proteins (antibodies) on hydrophobic and hydrophilic diamond surfaces. A powerful combination of time resolved impedance spectroscopy and data modeling with equivalent circuits allow a detailed insight in the protein behavior at an interface. It is found that hydrogenated diamond is greatly favorable for impedimetric read-out but causes slight conformational loss of the protein structure and therefore also its biological activity. The oxidized surface allows faster adsorption and a high biological activity but results in smaller impedimetric response
URI: http://hdl.handle.net/1942/13172
DOI: 10.1002/pssa.201100122
ISI #: 000295433600016
ISSN: 1862-6300
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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