www.uhasselt.be
DSpace

Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/13164

Title: Rapid assessment of the stability of DNA duplexes by impedimetric real-time monitoring of chemically induced denaturation
Authors: Van Grinsven, Bart
Vanden Bon, Natalie
Grieten, Lars
Murib, Mohammed Sharif
Janssens, S.D.
Haenen, Ken
Schneider, E.
Ingebrandt, S.
Schöning, M.J.
Vermeeren, Veronique
Ameloot, Marcel
Michiels, Luc
Thoelen, Ronald
De Ceuninck, Ward
Wagner, Patrick
Issue Date: 2011
Citation: LAB ON A CHIP, 2011(9), p. 1656-1663
Abstract: In this article, we report on the electronic monitoring of DNA denaturation by NaOH using electrochemical impedance spectroscopy in combination with fluorescence imaging as a reference technique. The probe DNA consisting of a 36-mer fragment was covalently immobilized on nanocrystalline-diamond electrodes and hybridized with different types of 29-mer target DNA (complementary, single-nucleotide defects at two different positions, and a non-complementary random sequence). The mathematical separation of the impedimetric signals into the time constant for NaOH exposure and the intrinsic denaturation-time constants gives clear evidence that the denaturation times reflect the intrinsic stability of the DNA duplexes. The intrinsic time constants correlate with calculated DNA-melting temperatures. The impedimetric method requires minimal instrumentation, is label-free and fast with a typical time scale of minutes and is highly reproducible. The sensor electrodes can be used repetitively. These elements suggest that the monitoring of chemically induced denaturation at room temperature is an interesting approach to measure DNA duplex stability as an alternative to thermal denaturation at elevated temperatures, used in DNA-melting experiments and single nucleotide polymorphism (SNP) analysis.
URI: http://hdl.handle.net/1942/13164
DOI: 10.1039/C1LC20027E
ISI #: 000289363800012
ISSN: 1473-0197
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
N/A279.44 kBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.