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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/13119

Title: Contrasting colonization and plant growth promoting capacity between wild type and a gfp-derative of the endophyte Pseudomonas putida W619 in hybrid poplar
Authors: Weyens, Nele
Boulet, Jana
Adriaensen, Kristin
Timmermans, Jean-Pierre
Prinsen, Els
Van Oevelen, Sandra
D'Haen, Jan
Smeets, Karen
Van Der Lelie, Daniel
Taghavi, Safiyh
Vangronsveld, Jaco
Issue Date: 2012
Citation: PLANT AND SOIL, 356(1-2), p. 217-230
Abstract: Abstract This study aims to investigate the colonization of poplar by the endophyte Pseudomonas putida W619 and its capacity to promote plant growth. Poplar cuttings were inoculated with P. putida W619 (wild-type or gfp-labelled). The colonization of both strains was investigated and morphological, physiological and biochemical parameters were analyzed to evaluate plant growth promotion. Inoculation with P. putida W619 (wild-type) resulted in remarkable growth promotion, decreased activities of antioxidative defence related enzymes, and reduced stomatal resistance, all indicative of improved plant health and growth in comparison with the noninoculated cuttings. In contrast, inoculation with gfplabelled P. putida W619 did not promote growth; it even had a negative effect on plant health and growth. Furthermore, compared to the wildtype strain, colonization by the gfp-labelled P. putida W619::gfp1 was much lower; it only colonized the rhizosphere and root cortex while the wild-type strain also colonized the root xylem vessels. Despite the strong plant growth promoting capacity of P. putida W619 (wild-type), after gfp labelling its growth promoting characteristics disappeared and its colonization capacity was strongly influenced; for these reasons gfp labelling should be applied with sufficient caution.
URI: http://hdl.handle.net/1942/13119
DOI: 10.1007/s11104-011-0831-x
ISI #: 000305528500017
ISSN: 0032-079X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2013
Appears in Collections: Research publications

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