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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12566

Title: Minocycline-conditioning brings surveying and reactive microglial cells to an alerted state according to their potassium channel profile
Authors: Dries, Eef
Advisors: BRONE, Bert
RIGO, Jean-Michel
Issue Date: 2010
Publisher: tUL Diepenbeek
Abstract: Microglial cells, the macrophages of the central nervous system (CNS), are key players during inflammatory processes in the CNS and are characterized by the expression of several types of voltage-gated potassium (Kv) channels, such as the delayed rectifying potassium (Kdr) current in reactive microglial cells. This Kdr current has been reported as a physiological marker for microglial activation and is an important target to control microglial activities. In this study we investigated whether minocycline, a known microglial activation inhibitor, was able to inhibit the functional expression of this Kdr current. Therefore, the I/V relationships and the function of the Kv channels of primary cultured rat microglial cells were measured using the whole-cell patch-clamp technique. The I/V profiles were compared in control and lipopolysaccharide (LPS)-activated (100 ng/ml) microglial cells in absence and presence of minocycline (400 µg/ml).
Notes: master in de biomedische wetenschappen-klinische moleculaire wetenschappen
URI: http://hdl.handle.net/1942/12566
Category: T2
Type: Theses and Dissertations
Appears in Collections: Master theses

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