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|Title: ||Reusable chromium-coated quartz crystal microbalance for immunosensing|
|Authors: ||Oliver, M. J.|
Sanchez-Rojas, J. L.
|Issue Date: ||2011|
|Publisher: ||ELSEVIER SCIENCE BV|
|Citation: ||COLLOIDS AND SURFACES B-BIOINTERFACES, 88(1). p. 191-195|
|Abstract: ||The application of oxidized chromium as a reusable platform for the development of immunosensors is presented. Chromium films were deposited on quartz crystal microbalances to study the affinity interaction between rabbit immunoglobulin G (IgG) and goat anti-rabbit IgG. A covalent approach, based on the silane chemistry, was followed for the grafting of either the rabbit IgG or the anti-rabbit IgG on the silane-modified chromium surface. Next the differences between the deposition of rabbit IgG on immobilized anti-rabbit IgG and the deposition of anti-rabbit IgG on immobilized rabbit IgG were investigated. The chromium layer could be reused between experiments, after the proper removal of the organic layers with piranha etch, obtaining a high repeatability in the steps of the functionalization protocol. (C) 2011 Elsevier B.V. All rights reserved.|
|Notes: ||[Oliver, MJ; Hernando-Garcia, J; Sanchez-Rojas, JL] Univ Castilla La Mancha, Dept Ingn Elect Elect Automat & Comunicac, ETSI Ind, E-13071 Ciudad Real, Spain. [Pobedinskas, P; Haenen, K] Hasselt Univ, IMO, B-3590 Diepenbeek, Belgium. [Haenen, K] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Rios, A] Univ Castilla La Mancha, Dept Quim Analit & Tecnol Alimentos, Fac Quim, E-13071 Ciudad Real, Spain.
|ISI #: ||000295344800025|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2012|
|Appears in Collections: ||Research publications|
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