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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12354

Title: Reusable chromium-coated quartz crystal microbalance for immunosensing
Authors: Oliver, M. J.
Hernando-Garcia, J.
POBEDINSKAS, Paulius
HAENEN, Ken
Rios, A.
Sanchez-Rojas, J. L.
Issue Date: 2011
Publisher: ELSEVIER SCIENCE BV
Citation: COLLOIDS AND SURFACES B-BIOINTERFACES, 88(1). p. 191-195
Abstract: The application of oxidized chromium as a reusable platform for the development of immunosensors is presented. Chromium films were deposited on quartz crystal microbalances to study the affinity interaction between rabbit immunoglobulin G (IgG) and goat anti-rabbit IgG. A covalent approach, based on the silane chemistry, was followed for the grafting of either the rabbit IgG or the anti-rabbit IgG on the silane-modified chromium surface. Next the differences between the deposition of rabbit IgG on immobilized anti-rabbit IgG and the deposition of anti-rabbit IgG on immobilized rabbit IgG were investigated. The chromium layer could be reused between experiments, after the proper removal of the organic layers with piranha etch, obtaining a high repeatability in the steps of the functionalization protocol. (C) 2011 Elsevier B.V. All rights reserved.
Notes: [Oliver, MJ; Hernando-Garcia, J; Sanchez-Rojas, JL] Univ Castilla La Mancha, Dept Ingn Elect Elect Automat & Comunicac, ETSI Ind, E-13071 Ciudad Real, Spain. [Pobedinskas, P; Haenen, K] Hasselt Univ, IMO, B-3590 Diepenbeek, Belgium. [Haenen, K] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Rios, A] Univ Castilla La Mancha, Dept Quim Analit & Tecnol Alimentos, Fac Quim, E-13071 Ciudad Real, Spain. MariaJesus.Oliver@uclm.es
URI: http://hdl.handle.net/1942/12354
DOI: 10.1016/j.colsurfb.2011.06.030
ISI #: 000295344800025
ISSN: 0927-7765
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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