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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12332

Title: Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish human biomonitoring program FLEHS II
Authors: Croes, K.
Van Langenhove, K.
Den Hond, E.
BRUCKERS, Liesbeth
Colles, A.
Koppen, G.
Loots, I.
Nelen, V.
Schoeters, G.
Van Larebeke, N.
Denison, M. S.
Vandermarken, T.
Elskens, M.
Baeyens, W.
Issue Date: 2011
Citation: TALANTA, 85(5). p. 2484-2491
Abstract: Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the throughput analysis of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. In this study, a method for the separate analysis of PCDD/Fs and dioxin-like PCBs (dl-PCBs) in human serum with the new sensitive H1L7.5c1 mouse hepatoma cell line was optimized. Sample dilution factors of 5 and 2.4 were selected for routine analysis of respectively the PCDD/Fs and dl-PCBs. The validation studies showed that repeatability and within-lab reproducibility for the quality control (QC) standard were within the in-house criteria. A long-term within-lab reproducibility of 25% for the PCDD/F fraction and 41% for the dl-PCB fraction for the analysis of pooled serum samples, expressed as pg BEQ/g fat, was determined. CALUX recoveries of the spiked procedural blanks were within the acceptable in-house limits of 80-120% for both fractions and the LOQ was 30.3 pg BEQ/g fat for the PCDD/Fs and 14.5 pg BEQ/g fat for the dl-PCBs. The GC-HRMS recovery of a C13-spiked pooled serum sample was between 60 and 90% for all PCDD/F congeners and between 67 and 82% for the non-ortho PCBs. An adequate separation between both fractions was found. The CALUX/GC-HRMS ratio for a pooled serum sample was respectively 2.0 and 1.4 for the PCDD/Fs and the dl-PCBs, indicating the presence of additional AhR active compounds. As expected, a correlation was found between human serum samples analyzed with both the new H1L7.5c1 cell line and the more established H1L6.1c3 cell line. The geometric mean CALUX-BEQ values, reported for the adolescents of the second Flemish Environment and Health Study (FLEHS II) recruited in 2009-2010, were 108(95% CI: 101-114) pg CALUX-BEQ/g fat for the PCDD/Fs and 32.1 (30.1-34.2) pg CALUX-BEQ/g fat for the dioxin-like PCBs. 2011 Elsevier By. All rights reserved.
Notes: [Croes, K; Van Langenhove, K; Vandermarken, T; Elskens, M; Baeyens, W] Vrije Univ Brussel, Dept Analyt & Environm Chem ANCH, B-1050 Brussels, Belgium. [Den Hond, E; Colles, A; Koppen, G; Schoeters, G] Flemish Inst Technol Res Vito Environm Hlth & Res, Mol, Belgium. [Bruckers, L] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. [Loots, I] Univ Antwerp, B-2020 Antwerp, Belgium. [Nelen, V] Prov Inst Hyg, Antwerp, Belgium. [Nawrot, T] Katholieke Univ Leuven, Unit Lung Toxicol, Louvain, Belgium. [Nawrot, T] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium. [Croes, K; Van Larebeke, N] Ghent Univ Hosp, Study Ctr Carcinogenesis & Primary Prevent Canc, B-9000 Ghent, Belgium. [Denison, MS] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA. kim.croes@vub.ac.be
URI: http://hdl.handle.net/1942/12332
DOI: 10.1016/j.talanta.2011.07.103
ISI #: 000296115600033
ISSN: 0039-9140
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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