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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12234

Title: Microbiological and Molecular Assessment of Bacteriophage ISP for the Control of Staphylococcus aureus
Authors: Vandersteegen, Katrien
Mattheus, Wesley
Ceyssens, Pieter-Jan
Bilocq, Florence
De Vos, Daniel
Pirnay, Jean-Paul
NOBEN, Jean-Paul
Merabishvili, Maia
Lipinska, Urszula
Hermans, Katleen
Lavigne, Rob
Issue Date: 2011
Citation: PLOS ONE, 6 (9)
Abstract: The increasing antibiotic resistance in bacterial populations requires alternatives for classical treatment of infectious diseases and therefore drives the renewed interest in phage therapy. Methicillin resistant Staphylococcus aureus (MRSA) is a major problem in health care settings and live-stock breeding across the world. This research aims at a thorough microbiological, genomic, and proteomic characterization of S. aureus phage ISP, required for therapeutic applications. Host range screening of a large batch of S. aureus isolates and subsequent fingerprint and DNA microarray analysis of the isolates revealed a substantial activity of ISP against 86% of the isolates, including relevant MRSA strains. From a phage therapy perspective, the infection parameters and the frequency of bacterial mutations conferring ISP resistance were determined. Further, ISP was proven to be stable in relevant in vivo conditions and subcutaneous as well as nasal and oral ISP administration to rabbits appeared to cause no adverse effects. ISP encodes 215 gene products on its 138,339 bp genome, 22 of which were confirmed as structural proteins using tandem electrospray ionization-mass spectrometry (ESI-MS/MS), and shares strong sequence homology with the 'Twort-like viruses'. No toxic or virulence-associated proteins were observed. The microbiological and molecular characterization of ISP supports its application in a phage cocktail for therapeutic purposes.
Notes: [Vandersteegen, K; Mattheus, W; Ceyssens, PJ; Lavigne, R] Katholieke Univ Leuven, Div Gene Technol, B-3001 Heverlee, Belgium [Lipinska, U; Hermans, K] Univ Ghent, Dept Pathol Bacteriol & Poultry Dis, Merelbeke, Belgium [Noben, JP] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium [Noben, JP] Transnat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium [Bilocq, F; De Vos, D; Pirnay, JP; Merabishvili, M] Queen Astrid Mil Hosp, Burn Ctr, Lab Mol & Cellular Technol, Brussels, Belgium [Merabishvili, M] Eliava Inst Bacteriophage Microbiol & Virol, Tbilisi, Rep of Georgia rob.lavigne@biw.kuleuven.be
URI: http://hdl.handle.net/1942/12234
DOI: 10.1371/journal.pone.0024418
ISI #: 000294803100030
ISSN: 1932-6203
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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