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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12135

Title: Impedimetric, diamond-based immmunosensor for the detection of C-reactive protein
Authors: VERMEEREN, Veronique
BIJNENS, Nathalie
WAGNER, Patrick
Issue Date: 2011
Citation: SENSORS AND ACTUATORS B-CHEMICAL, 157(1). p. 130-138
Abstract: The high prevalence of cardiovascular diseases (CVD) demands a reliable and sensitive risk assessment technique. In order to develop a fast and label-free immunosensor for C-reactive protein (CRP), a risk factor for this condition, anti-CRP antibodies were physically adsorbed to the hydrogen (H)-terminated surface of nanocrystalline diamond (NCD). An Enzyme-Linked ImmunoSorbent Assay (ELISA) reference technique showed that this was a suitable substrate for antibody-antigen recognition reactions. Electrochemical Impedance Spectroscopy (EIS) was used to electronically detect CRP recognition. The specificity of the immunosensor was demonstrated by incubation with CRP and plasminogen as reference molecule. A different impedance behavior was observed in real-time after CRP addition as compared to plasminogen addition: the impedance increased only during CRP incubation. Fitting the data showed that this corresponded with a decrease in capacitance of the molecular layer due to its increased thickness by specific CRP recognition. Sensitivity experiments in real-time showed a clear discrimination between 1 mu M,100 nM, and 10 nM of CRP after 10 min at 100 Hz. Since, 10 nM of CRP was still clearly distinguishable from buffer solution, our CRP-directed immunosensor prototype reaches a sensitivity that is within the physiologically relevant concentration range of this biomarker in healthy controls and CVD patients. Moreover, this prototype displayed real-time discriminating power between spiked and unspiked serum, and thus also shows its applicability in this biological matrix. (C) 2011 Elsevier B.V. All rights reserved.
Notes: Michiels, L (reprint author) [Vermeeren, V; Grieten, L; Vanden Bon, N; Bijnens, N; Wenmackers, S; Janssens, SD; Haenen, K; Wagner, P; Michiels, L] Hasselt Univ, B-3590 Diepenbeek, Belgium [Vermeeren, V; Vanden Bon, N; Michiels, L] Transnat Univ Limburg, Sch Life Sci, Biomed Res Inst, B-3590 Diepenbeek, Belgium [Grieten, L; Bijnens, N; Wenmackers, S; Janssens, SD; Haenen, K; Wagner, P] Transnat Univ Limburg, Sch Life Sci, Inst Mat Res, B-3590 Diepenbeek, Belgium [Haenen, K] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium luc.michiels@uhasselt.be
URI: http://hdl.handle.net/1942/12135
DOI: 10.1016/j.snb.2011.03.037
ISI #: 000293436900019
ISSN: 0925-4005
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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