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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12109

Title: Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide
Authors: BOHRN, Ulrich
Stuetz, Evamaria
Fleischer, Maximilian
Schoening, Michael J.
WAGNER, Patrick
Issue Date: 2011
Abstract: In the past years, whole cell gas toxicity assays have been developed in order to study the harmful effects of gaseous chemicals. Cells are incubated with nutrition medium supplemented with the gaseous substances to be tested. These methods are not feasible for the examination of toxic gases, which are insoluble in water. Carbon monoxide (CO), a poorly soluble and toxic gas (lethal dose 1 vol% for some minutes) has been chosen as a model gas. Besides its binding to hemoglobin, it also leads to an inhibition of cytochrome c oxidase in the mitochondrial respiratory chain of living cells. Thus, a decrease in the respiratory behavior of eukaryotic cells indicates the presence of CO in a gaseous phase. Based on the Bionas (R) 2500 analyzing system, a device to measure the metabolic and morphological effects, caused by direct gas exposure on eukaryotic cells was established. The gaseous substances directly contact the eukaryotic cell line V79 (Chinese hamster lung fibroblast) without interfering liquid phase in between. Beside the measurement of oxygen consumption also acidification and impedance changes of the cell culture are detected by a metabolic sensor chip. In order to increase the stability different chip surface coatings like collagen A and poly-L-lysin (PLL) were used Adhesion coating leads to a tighter junction of the cells to the sensor chip surface and thus enables higher stability of the confluent cell monolayer towards the gaseous flow during the gas exposure. The method reported here has the potential to become a valuable means for rapid monitoring of toxic compounds in gaseous phases. (C) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Notes: [Bohrn, U; Wagner, P] Univ Hasselt, Inst Mat Res IMO IMOMEC, B-3590 Diepenbeek, Belgium [Bohrn, U; Stutz, E; Fleischer, M] Siemens AG, Corp Res & Technol, D-81379 Munich, Germany [Bohrn, U; Schoning, MJ] Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany ulrich.bohrn.ext@siemens.com
URI: http://hdl.handle.net/1942/12109
DOI: 10.1002/pssa.201000924
ISI #: 000292945800023
ISSN: 1862-6300
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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