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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12075

Title: Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part II: responses in the leaves and general conclusions
Authors: VANHOUDT, Nathalie
Horemans, Nele
Bello, Daniel Martinez
Havaux, Michel
Wannijn, Jean
Van Hees, May
Vandenhove, Hildegarde
Issue Date: 2011
Abstract: The cellular redox balance seems an important modulator under heavy metal stress. While for other heavy metals these processes are well studied, oxidative stress related responses are also known to be triggered under uranium stress but information remains limited. This study aimed to further unravel the mechanisms by which plants respond to uranium stress. Seventeen-day-old Arabidopsis thaliana seedlings, grown on a modified Hoagland solution under controlled conditions, were exposed to 0, 0.1, 1, 10 and 100 mu M uranium for 1,3 and 7 days. While in Part I of this study oxidative stress related responses in the roots were discussed, this second Part II discusses oxidative stress related responses in the leaves and general conclusions drawn from the results of the roots and the leaves will be presented. As several responses were already visible following 1 day exposure, when uranium concentrations in the leaves were negligible, a root-to-shoot signaling system was suggested in which plastids could be important sensing sites. While lipid peroxidation, based on the amount of thiobarbituric acid reactive compounds, was observed after exposure to 100 mu M uranium, affecting membrane structure and function, a transient concentration dependent response pattern was visible for lipoxygenase initiated lipid peroxidation. This transient character of uranium stress responses in leaves was emphasized by results of lipoxygenase (LOX2) and antioxidative enzyme transcript levels, enzyme capacities and glutathione concentrations both in time as with concentration. The ascorbate redox balance seemed an important modulator of uranium stress responses in the leaves as in addition to the previous transient responses, the total ascorbate concentration and ascorbate/dehydroascorbate redox balance increased in a concentration and time dependent manner. This could represent either a slow transient response or a stable increase with regard to plant acclimation to uranium stress. (C) 2011 Elsevier Ltd. All rights reserved.
Notes: [Vanhoudt, N; Horemans, N; Wannijn, J; Van Hees, M; Vandenhove, H] Belgian Nucl Res Ctr SCK CEN, B-2400 Mol, Belgium [Vanhoudt, N; Cuypers, A; Remans, T; Opdenakker, K; Smeets, K; Vangronsveld, J] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium [Bello, DM] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, B-3590 Diepenbeek, Belgium [Havaux, M] CEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, France
URI: http://hdl.handle.net/1942/12075
DOI: 10.1016/j.jenvrad.2011.03.013
ISI #: 000292015700014
ISSN: 0265-931X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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