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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11868

Title: In vivo electroporation of the central nervous system: A non-viral approach for targeted gene delivery
Authors: De Vry, Jochen
Martinez-Martinez, Pilar
Losen, Mario
Temel, Yasin
Steckler, Thomas
Steinbusch, Harry W. M.
DE BAETS, Marc
Prickaerts, Jos
Issue Date: 2010
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: PROGRESS IN NEUROBIOLOGY, 92 (3). p. 227-244
Abstract: Electroporation is a widely used technique for enhancing the efficiency of DNA delivery into cells. Application of electric pulses after local injection of DNA temporarily opens cell membranes and facilitates DNA uptake. Delivery of plasmid DNA by electroporation to alter gene expression in tissue has also been explored in vivo. This approach may constitute an alternative to viral gene transfer, or to transgenic or knock-out animals. Among the most frequently electroporated target tissues are skin, muscle, eye, and tumors. Moreover, different regions in the central nervous system (CNS), including the developing neural tube and the spinal cord, as well as prenatal and postnatal brain have been successfully electroporated. Here, we present a comprehensive review of the literature describing electroporation of the CNS with a focus on the adult brain. In addition, the mechanism of electroporation, different ways of delivering the electric pulses, and the risk of damaging the target tissue are highlighted. Electroporation has been successfully used in humans to enhance gene transfer in vaccination or cancer therapy with several clinical trials currently ongoing. Improving the knowledge about in vivo electroporation will pave the way for electroporation-enhanced gene therapy to treat brain carcinomas, as well as CNS disorders such as Alzheimer's disease, Parkinson's disease, and depression. (C) 2010 Elsevier Ltd. All rights reserved.
Notes: Prickaerts, J (reprint author), Maastricht Univ, Sch Mental Hlth & Neurosci, Dept Psychiat & Neuropsychol, POB 616, NL-6200 MD Maastricht, Netherlands. [De Vry, Jochen; Martinez-Martinez, Pilar; Losen, Mario; Temel, Yasin; Steinbusch, Harry W. M.; De Baets, Marc H.; Prickaerts, Jos] Maastricht Univ, Sch Mental Hlth & Neurosci, Dept Psychiat & Neuropsychol, NL-6200 MD Maastricht, Netherlands. [De Vry, Jochen; Martinez-Martinez, Pilar; Losen, Mario; Temel, Yasin; Steinbusch, Harry W. M.; De Baets, Marc H.; Prickaerts, Jos] Maastricht Univ, European Grad Sch Neurosci EURON, NL-6200 MD Maastricht, Netherlands. [Temel, Yasin] Maastricht Univ, Med Ctr, Dept Neurosurg, NL-6200 MD Maastricht, Netherlands. [Steckler, Thomas] Johnson & Johnson PRD, Dept Psychiat, Beerse, Belgium. [De Baets, Marc H.] Hasselt Univ, Translationale Univ Limburg, Hasselt, Belgium. [De Baets, Marc H.] Biomed Res Inst, Sch Life Sci, Hasselt, Belgium. jos.prickaerts@maastrichtuniversity.nl
URI: http://hdl.handle.net/1942/11868
DOI: 10.1016/j.pneurobio.2010.10.001
ISI #: 000285661800001
ISSN: 0301-0082
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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