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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11818

Title: Endophytes and Their Potential to Deal with Co-Contamination of Organic Contaminants (Toluene) and Toxic Metals (Nickel) During Phytoremediation
Authors: Weyens, Nele
Truyens, Sascha
Saenen, Eline
Boulet, Jana
Dupae, Joke
Taghavi, Safiyh
Van Der Lelie, Daniël
Carleer, Robert
Vangronsveld, Jaco
Issue Date: 2011
Citation: INTERNATIONAL JOURNAL OF PHYTOREMEDIATION, 13(3), (ART N° PII 927134237). p. 244-255
Abstract: The aim was to investigate if engineered endophytes that are capable of degrading organic contaminants, and deal with or ideally improve uptake and translocation of toxic metals, can improve phytoremediation of mixed organic-metal pollution. As a model system, yellow lupine was inoculated with the endophyte Burkholderia cepacia VM1468 possessing (a) the pTOM-Bu61 plasmid, coding for constitutive toluene/TCE degradation, and (b) the chromosomally inserted ncc-nre Ni resistance/sequestration system. As controls, plants were inoculated with B. vietnamiensis BU61 (pTOM-Bu61) and B. cepacia BU72 (containing the ncc-nre Ni resistance/sequestration system). Plants were exposed to mixes of toluene and Ni. Only inoculation with B. cepacia VM1468 resulted in decreased Ni and toluene phytotoxicity, as measured by a protective effect on plant growth and decreased activities of enzymes involved in antioxidative defence (catalase, guaiacol peroxidase, superoxide dismutase) in the roots. Besides, plants inoculated with B. cepacia VM1468 and B. vietnamiensis BU61 released less toluene through the leaves than non-inoculated plants and those inoculated with B. cepacia BU72. Ni-uptake in roots was slightly increased for B. cepacia BU72 inoculated plants. These results indicate that engineered endophytes have the potential to assist their host plant to deal with co-contamination of toxic metals and organic contaminants during phytoremediation.
Notes: [Weyens, Nele; Truyens, Sascha; Saenen, Eline; Boulet, Jana; Dupae, Joke; Carleer, Robert; Vangronsveld, Jaco] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Taghavi, Safiyh; van der Lelie, Daniel] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. jaco.vangronsveld@uhasselt.be
URI: http://hdl.handle.net/1942/11818
DOI: 10.1080/15226511003753920
ISI #: 000286821900003
ISSN: 1522-6514
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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