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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11779

Title: Validity of oral mucosal transudate specimens for HIV testing using enzyme-linked immunosorbent assay in children in Chimanimani district, Zimbabwe
Authors: Mashange, Wilson
Gwini, Stella May
Mahati, Stanford T.
Buzuzi, Stephen S.
Munyati, Shungu
Chandiwana, Brian
Rusakaniko, Simbarashe
Gomo, Exneyia
Issue Date: 2011
Citation: SAMJ SOUTH AFRICAN MEDICAL JOURNAL, 101(1). p. 49-52
Abstract: Objective. To assess the validity of oral mucosal transudate (OMT) specimens for HIV testing in children using enzyme-linked immunosorbent assay (ELISA). Methods. A cross-sectional descriptive study was conducted as part of a community-based behavioural and HIV sero-status survey of adults and children in the Chimanimani district of Zimbabwe. Dried blood spot (DBS) and OMT samples were collected from children aged between 2 and 14 years, inclusive. Both samples were tested for HIV using the Vironostika Uniform II plus O kits. The main study outcomes were the sensitivity and specificity of OMT samples, with DBS as the gold-standard specimen. Results. Paired DBS and OMT specimens were available from 1 274 (94.496) of the 1 350 children enrolled. Using the DBS, HIV prevalence was 3.2%. Overall sensitivity of OMT was 48.8% (95% confidence interval (Cl) 33.3 - 64.5), and specificity was 98.5% (95% Cl 97.7 - 99.1). Conclusion. The overall sensitivity of OMT specimens for HIV testing in children using ELISA was low. Stratifying the analysis by sector showed that OMT samples are good specimens for HIV testing. It is important to note that factors such as the low HIV prevalence in our study population, quality of the OMT, diet and oral hygiene could have influenced the results.
Notes: [Gwini, Stella May] Monash Univ, Clayton, Vic 3800, Australia. [Mahati, Stanford T.] Univ Witwatersrand, Sch Social Sci, Johannesburg, South Africa. [Mutambanengwe, Chenjerai K.] Hasselt Univ, Diepenbeek, Belgium. [Rusakaniko, Simbarashe] Univ Zimbabwe, Coll Hlth Sci, Dept Community Med, Harare, Zimbabwe. [Gomo, Exneyia] Univ Malawi, Coll Med, Res Support Ctr, Zomba, Malawi. [Gomo, Exneyia] Univ Zimbabwe, Coll Hlth Sci, Dept Immunol, Zomba, Malawi. wmashange@yahoo.co.uk
URI: http://hdl.handle.net/1942/11779
ISI #: 000287165400021
ISSN: 0256-9574
Category: A1
Type: Journal Contribution
Validation: ecoom, 2012
Appears in Collections: Research publications

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