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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11564

Title: Effect of pH on the stability of kidney injury molecule 1 (KIM-1) and on the accuracy of its measurement in human urine
Authors: Pennemans, Valerie
De Winter, Liesbeth
Faes, Christel
Van Kerkhove, Emmy
Reynders, Carmen
Rigo, Jean-Michel
Swennen, Quirine
Penders, Joris
Issue Date: 2010
Citation: CLINICA CHIMICA ACTA, 411(23-24). p. 2083-2086
Abstract: Background: Urinary KIM-1 is a novel biomarker for tubular kidney damage, however little is known about its stability. The goal of this study is to examine the effect of urinary pH on the stability of KIM-1. Methods: Urine samples were collected from 45 volunteers. Samples were aliquoted, adapted to different pH values (range 4 to 9) and stored at -80 degrees C. After thawing, each aliquot was divided into two, of which one was used to measure KIM-1 (human tim-1/kim-1/Havcr Elisa kit; R&D systems) at the same pH at which it was stored, while the other was readapted to pH 7 before measurement. Results: KIM-1 values of aliquots of the same sample are stable when stored at pH 6.7 and 8 whereas at lower and higher storage pH, KIM-1 levels decrease significantly. When samples are readjusted to a neutral pH just before KIM-1 measurement, there are no longer significant differences between KIM-1 in aliquots stored at different pH values. Conclusions: No effect of urinary pH on the stability of KIM-1 was seen. However, the only commercially available human tim-1/kim-1/Havcr Elisa kit of RD systems is pH dependent and we therefore suggest samples should be adjusted to neutral pH before measurement. (C) 2010 Elsevier B.V. All rights reserved.
Notes: [Pennemans, Valerie; De Winter, Liesbeth M.; Rigo, Jean-Michel; Swennen, Quirine; Penders, Joris] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Pennemans, Valerie; De Winter, Liesbeth M.; Van Kerkhove, Emmy; Rigo, Jean-Michel; Swennen, Quirine; Penders, Joris] Transnat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [Reynders, Carmen; Penders, Joris] Ziekenhuis Oost Limburg ZOL, Dept Biol Clin, Genk, Belgium. [Van Kerkhove, Emmy] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium. [Faes, Christel] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. valerie.pennemans@uhasselt.be
URI: http://hdl.handle.net/1942/11564
DOI: 10.1016/j.cca.2010.09.008
ISI #: 000283916600041
ISSN: 0009-8981
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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