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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11473

Title: Proteome Analysis of Multiple Compartments in a Mouse Model of Chemical-Induced Asthma
Authors: HAENEN, Steven
Vanoirbeek, Jeroen A. J.
De Vooght, Vanessa
Maes, Evelyne
Schools, Liliane
Nemery, Benoit
Hoet, Peter H. M.
CLYNEN, Elke
Issue Date: 2010
Publisher: AMER CHEMICAL SOC
Citation: JOURNAL OF PROTEOME RESEARCH, 9 (11). p. 5868-5876
Abstract: Occupational asthma is the principal cause of work-related respiratory disease in the industrial world. Toluene-2,4-diisocyanate (TDI) is one of the most common respiratory sensitizers leading to occupational asthma. Using a mouse model of chemical-induced asthma, we explored proteome changes in multiple compartments of mice sensitized and challenged with TDI or acetone-olive oil (AOO; vehicle). Airway reactivity to methacholine and a bronchoalveolar lavage (BAL) cell count was assessed in treated and control mice, 1 day after challenge. Subsequently, two-dimensional differential gel electrophoresis (2D-DIGE) was performed on auricular lymph nodes, BAL, and serum comparing TDI-treated and vehicletreated control mice. The differentially expressed proteins were identified by mass spectrometry and pathway analysis was performed. TDI-treated mice exhibit increased airway reactivity (2.6-fold increase) and a neutrophilic inflammation in the BAL fluid, compared to control mice. 2D-DIGE showed 53, 210, and 40 differentially expressed proteins in the auricular lymph nodes, BAL, and serum of TDI-treated versus vehicle-treated mice, respectively. Several of the identified proteins could be linked with inflammation, neutrophil chemotaxis, and/or oxidative stress. Physiologic and immunologic readouts of the asthmatic phenotype, such as inflammation, were confirmed in three compartments by several of the differentially expressed proteins via 2D-DIGE and computerized pathway analysis.
Notes: [Haenen, Steven; Vanoirbeek, Jeroen A. J.; De Vooght, Vanessa; Nemery, Benoit; Hoet, Peter H. M.] Katholieke Univ Leuven, Res Unit Lung Toxicol, B-3000 Louvain, Belgium. [Haenen, Steven; Maes, Evelyne; Schools, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, B-3000 Louvain, Belgium. [Clynen, Elke] Hasselt Univ, BIOMED Res Inst, B-3590 Diepenbeek, Belgium. peter.hoet@med.kuleuven.be
URI: http://hdl.handle.net/1942/11473
DOI: 10.1021/pr100638m
ISI #: 000283810500035
ISSN: 1535-3893
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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