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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11306

Title: A novel method for making human monoclonal antibodies
Authors: FRAUSSEN, Judith
Vrolix, K.
Martinez-Martinez, Pilar
Losen, M.
Meulemans, E.
SOMERS, Veerle
Issue Date: 2010
Citation: JOURNAL OF AUTOIMMUNITY, 35 (2). p. 130-134
Abstract: We have developed a B cell immortalization method for low B cell numbers per well using simultaneous B cell stimulation by CpG2006 and B cell infection by Epstein-Barr virus (EBV), followed by an additional CpG2006 and interleukin-2 (IL-2) stimulus. Using this method, immunoglobulin G (IgG)-producing immortalized B cell lines were generated from peripheral blood IgG(+)CD22(+) B cells with an efficiency of up to 83%. Antibody can already be obtained from the culture supernatant after 3-4 weeks. Moreover, clonality analysis demonstrated monoclonality in 87% of the resulting immortalized B cell lines. Given the high immortalization efficiency and monoclonality rate, evidence is provided that no further sub-cloning is necessary. An important application of this B cell immortalization method is the characterization of (autoreactive) antibodies from patients with autoimmune disease. This could eventually lead to the identification of new autoantigens, disease markers or targets for therapy. (C) 2010 Elsevier Ltd. All rights reserved.
Notes: [Fraussen, J.; De Baets, M. H.; Stinissen, P.; Somers, V.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Fraussen, J.; De Baets, M. H.; Stinissen, P.; Somers, V.] Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [Vrolix, K.; Martinez-Martinez, P.; Losen, M.; De Baets, M. H.] Maastricht Univ, Sch Mental Hlth & Neurosci, Dept Neurosci, Maastricht, Netherlands. [Meulemans, E.] Univ Limburg, Acad Hosp Maastricht, Dept Pathol, Maastricht, Netherlands. veerle.somers@uhasselt.be
URI: http://hdl.handle.net/1942/11306
DOI: 10.1016/j.jaut.2010.05.001
ISI #: 000281986100004
ISSN: 0896-8411
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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