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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11140

Title: Characterization of MabA, a modulator of Lactobacillus rhamnosus GG adhesion and biofilm formation
Authors: Velez, Monica Perea
Petrova, Mariya I.
Lebeer, Sarah
Verhoeven, Tine L. A.
Claes, Ingmar
Tynkkynen, Soile
Vanderleyden, Jos
De Keersmaecker, Sigrid C. J.
Issue Date: 2010
Abstract: The probiotic Lactobacillus rhamnosus GG, first isolated from healthy human gut microbiota, has been reported to adhere very well to components of the intestinal mucosa, thereby enabling transient colonization of the gastrointestinal tract (GIT). In a search for the genes responsible for the good adherence capacity of this strain, a genomic region encoding a protein with homology to putative adhesion proteins (LGG_01865) and its putative regulator (LGG_01866) was identified. The sequence of the L. rhamnosus GG LGG_01865 encodes a polypeptide of 2419 amino acid residues containing 26 repetitive DUF1542 domains and a C-terminal LPxTG cell wall-anchoring motif. Phenotypic analyses of a dedicated LGG_01865 knockout mutant revealed a reduced biofilm formation capacity on abiotic surfaces and decreased adhesion to intestinal epithelial cells and tissues of the murine GIT. This suggests a modulating role for LGG_01865 in L. rhamnosus GG-host interactions. Therefore, we propose a new name for LGG_01865, i.e. MabA, modulator of adhesion and biofilm. Expression analysis indicated that LGG_01866 plays a conditional role in the regulation of LGG_01865 expression, i.e. when cells are grown under conditions of sugar starvation.
Notes: [Velez, Monica Perea; Petrova, Mariya I.; Lebeer, Sarah; Verhoeven, Tine L. A.; Claes, Ingmar; Vanderleyden, Jos; De Keersmaecker, Sigrid C. J.] Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Leuven, Belgium. [Lambrichts, Ivo] Univ Hasselt, Biomed Res Inst, Histol Lab, Diepenbeek, Belgium. [Tynkkynen, Soile] Valio Ltd, Helsinki, Finland. sigrid.dekeersmaecker@biw.kuleuven.be
URI: http://hdl.handle.net/1942/11140
DOI: 10.1111/j.1574-695X.2010.00680.x
ISI #: 000279900400016
ISSN: 0928-8244
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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