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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11046

Title: A MIP-based biomimetic sensor for the impedimetric detection of histamine in different pH environments
Authors: BONGAERS, Evi
ALENUS, Jan
HOREMANS, Frederik
WEUSTENRAED, Ans
LUTSEN, Laurence
VANDERZANDE, Dirk
CLEIJ, Thomas
TROOSTERS,
Brummer, RJ
WAGNER, Patrick
Issue Date: 2010
Publisher: WILEY-V C H VERLAG GMBH
Citation: PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 207(4). p. 837-843
Abstract: The development of novel biosensors is a rapidly growing field. Substituting the biological receptor layer from the biosensor with a synthetic receptor opens the door for the development of biomimetic sensors that are chemically and physically inert, as opposed to the sensors containing biological recognition elements. Using molecularly imprinted polymers (MIPs) the specificity and affinity of biological receptors can be mimicked. In addition, a MIP-based sensor can measure in harsh environments. Histamine occurs in harsh environments in food and bodily fluids and is chosen as the target molecule for impedimetric detection: When 10 nM histamine is present in pH neutral environments, the impedance increases 45% with respect to the impedance of the sensor without histamine. Specificity is tested with respect to histidine. The influence of the pH on the performance of the sensor is tested. In a pH range of pH 5-12 the MIPs are stable, although they exhibit a varying degree of protonation. The same holds true for the target molecule of which the protonation also varies with the pH of the solution. It is shown that the pH dependent degree of protonation of both the MIP and the histamine has a large impact on the binding of histamine to the nanocavity in the MIP. Hence, the detection of histamine by a MIP-based sensor is affected by the PH of the solution. (C) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Notes: [Bongaers, E.; Alenus, J.; Horemans, F.; Weustenraed, A.; Vanderzande, D.; Cleij, T. J.; Wagner, P.] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Lutsen, L.; Vanderzande, D.; Wagner, P.] IMEC, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Troost, F. J.; Brummer, R. -J.] Maastricht Univ, NUTRIM, Dept Internal Med, NL-6229 ER Maastricht, Netherlands. Evi.Bongaers@uhasselt.be; Patrick.Wagner@uhasselt.be
URI: http://hdl.handle.net/1942/11046
DOI: 10.1002/pssa.200983307
ISI #: 000277565300011
ISSN: 1862-6300
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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