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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11025

Title: cDNA phage display as a novel tool to screen for cellular targets of chemical compounds
Authors: Van Dorst, Bieke
Mehta, Jaytry
Rouah-Martin, Elsa
SOMERS, Veerle
De Coen, Wim
Blust, Ronny
Robbens, Johan
Issue Date: 2010
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: TOXICOLOGY IN VITRO, 24(5). p. 1435-1440
Abstract: cDNA phage display is frequently used in drug development to screen for cellular target of drugs. However, in toxicology, cDNA phage display remains unexplored, although it has large potential in this field. In this study, cDNA phage display is demonstrated as a novel tool to screen for interactions between chemical compounds and cellular targets. The knowledge of these target interactions is valuable to have a more complete understanding of the mechanisms of action of chemical compounds. Bisphenol A (BPA) was selected as a model compound for this study. By selection of the cellular proteins that bind BPA with cDNA phage display, it was possible to identify a known cellular target of BPA, tubulin a and a possible novel cellular target of BPA, transforming acidic coiled-coil containing protein 3. Both these cellular proteins are involved in the mechanism of cell division. The disruption of cell division is a known non-genomic effect of BPA. Non-genomic effects are not mediated by differences in gene expression and therefore important mechanistic information might be missed with the widely used differential gene expression techniques for mode of action research. This cDNA phage display technique can provide important additional information about the interaction of chemical compounds with cellular targets that mediates these non-genomic actions and therefore gives complementary information to toxicogenomic studies to obtain a more complete understanding of the mechanism of action of chemical compounds. (C) 2010 Elsevier Ltd. All rights reserved.
Notes: [Van Dorst, Bieke; Mehta, Jaytry; Rouah-Martin, Elsa; De Coen, Wim; Blust, Ronny; Robbens, Johan] Univ Antwerp, Dept Biol, Lab Ecophysiol Biochem & Toxicol, B-2020 Antwerp, Belgium. [Van Dorst, Bieke; Mehta, Jaytry; Rouah-Martin, Elsa; Robbens, Johan] Inst Agr & Fisheries Res ILVO, B-8400 Oostende, Belgium. [Somers, Veerle] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [De Coen, Wim] European Chem Agcy ECHA, F-00120 Helsinki, Finland. Bieke.vandorst@ua.ac.be
URI: http://hdl.handle.net/1942/11025
DOI: 10.1016/j.tiv.2010.04.003
ISI #: 000279650600014
ISSN: 0887-2333
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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