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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11013

Title: The beta-adrenergic system is involved in the regulation of the expression of avian uncoupling protein in the chicken
Authors: Joubert, R.
SWENNEN, Quirine
Sibut, V.
Crochet, S.
Cailleau-Audouin, E.
Buyse, J.
Decuypere, E.
Wrutniak-Cabello, C.
Cabello, G.
Tesseraud, S.
Collin, A.
Coustard, S.M.
Issue Date: 2010
Citation: DOMESTIC ANIMAL ENDOCRINOLOGY, 38(2). p. 115-125
Abstract: Avian uncoupling protein (avUCP) is orthologous to UCP3, which is suggested to be involved in fatty acid metabolism and to limit the mitochondrial production of reactive oxygen species in mammals. In the chicken, the role and regulation of avUCP remain to be clarified. The aim of this study was to explore the control of avUCP expression by the beta-adrenergic system, known to be involved in avian thermoregulation and lipid utilization, and in UCP expression in mammals. Therefore, we measured the expression of avUCP mRNA and protein in the Pectoralis major muscle of chickens injected with the beta(2) agonist isoproterenol, and we investigated the potential pathways involved in the regulation of avUCP mRNA expression. Avian UCP mRNA expression was increased 7-fold 4 h after isoproterenol injection, leading to a tendency to a 40% increase in avUCP protein 24 h post-injection. This increase was preceded, 30 min after isoproterenol injection, by changes in the chicken thyroid status and in the muscular expression of PPAR alpha, PPAR beta/delta, and PPAR gamma coactivator-1 alpha (PGC-1 alpha). Moreover, the analysis of the avUCP promoter sequence suggested potential binding sites for PPARs and for thyroid hormone receptors. We also detected the activation of AMP-activated protein kinase, which has recently been reported to be involved in UCP3 regulation in mammals. This study presents for the first time evidence of beta-adrenergic control on avUCP messenger expression in chicken muscle and suggests the potential involvement of AMPK and several transcription factors in this regulation. (C) 2010 Elsevier Inc. All rights reserved.
Notes: [Joubert, R.; Coustard, S. Metayer; Sibut, V.; Crochet, S.; Cailleau-Audouin, E.; Tesseraud, S.; Collin, A.] INRA, Rech Avicoles UR83, F-37380 Nouzilly, France. [Swennen, Q.; Buyse, J.; Decuypere, E.] Katholieke Univ Leuven, Dept Biosyst, Lab Livestock Physiol Immunol & Genet Domest Anim, B-3001 Louvain, Belgium. [Swennen, Q.] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Sibut, V.] ITAVI, Unite Rech Avicoles, F-37380 Nouzilly, France. [Wrutniak-Cabello, C.; Cabello, G.] INRA, Differenciat Cellulaire & Croissance UMR866, F-34060 Montpellier, France. [Wrutniak-Cabello, C.; Cabello, G.] Univ Montpellier 1, F-34060 Montpellier, France. [Wrutniak-Cabello, C.; Cabello, G.] Univ Montpellier 2, F-34060 Montpellier, France.
URI: http://hdl.handle.net/1942/11013
DOI: 10.1016/j.domaniend.2009.08.002
ISI #: 000277928400006
ISSN: 0739-7240
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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