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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10981

Title: Low Current-driven Micro-electroporation Allows Efficient In Vivo Delivery of Nonviral DNA into the Adult Mouse Brain
Authors: De Vry, Jochen
Martinez-Martinez, Pilar
Losen, Mario
Bode, Gerard H.
Temel, Yasin
Steckler, Thomas
Steinbusch, Harry W. M.
DE BAETS, Marc
Prickaerts, Jos
Issue Date: 2010
Publisher: NATURE PUBLISHING GROUP
Citation: MOLECULAR THERAPY, 18 (6). p. 1183-1191
Abstract: Viral gene transfer or transgenic animals are commonly used technologies to alter gene expression in the adult brain, although these approaches lack spatial specificity and are time consuming. We delivered plasmid DNA locally into the brain of adult C57BL/6 mice in vivo by voltage-and current-controlled electroporation. The low current-controlled delivery of unipolar square wave pulses of 125 mu A with microstimulation electrodes at the injection site gave 16 times higher transfection rates than a voltage-controlled electroporation protocol with plate electrodes resulting in currents of about 400 mA. Transfection was restricted to the target region and no damage due to the electric pulses was found. Our current-controlled electroporation protocol indicated that the use of very low currents resulting in applied voltages within the physiological range of the membrane potential, allows efficient transfection of nonviral plasmid DNA. In conclusion, low current-controlled electroporation is an excellent approach for electroporation in the adult brain, i.e., gene function can be influenced locally at a high level with no mortality and minimal tissue damage.
Notes: [De Vry, Jochen; Martinez-Martinez, Pilar; Losen, Mario; Bode, Gerard H.; Temel, Yasin; Steinbusch, Harry W. M.; De Baets, Marc; Prickaerts, Jos] Maastricht Univ, Dept Psychiat & Neuropsychol, Sch Mental Hlth & Neurosci, NL-6200 MD Maastricht, Netherlands. [De Vry, Jochen; Martinez-Martinez, Pilar; Losen, Mario; Bode, Gerard H.; Temel, Yasin; Steinbusch, Harry W. M.; De Baets, Marc; Prickaerts, Jos] Maastricht Univ, European Grad Sch Neurosci EURON, NL-6200 MD Maastricht, Netherlands. [Temel, Yasin] Maastricht Univ, Med Ctr, Dept Neurosurg, NL-6200 MD Maastricht, Netherlands. [Steckler, Thomas] Johnson & Johnson PRD, Dept Psychiat, Beerse, Belgium. [De Baets, Marc] Hasselt Univ, Sch Life Sci, Biomed Res Inst, Hasselt, Belgium. [De Baets, Marc] Hasselt Univ, Translat Univ Limburg, Hasselt, Belgium. jos.prickaerts@np.unimaas.nl
URI: http://hdl.handle.net/1942/10981
DOI: 10.1038/mt.2010.62
ISI #: 000278545800016
ISSN: 1525-0016
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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