Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10979

Title: Genome Sequence of the Plant Growth Promoting Endophytic Bacterium Enterobacter sp 638
Authors: Taghavi, Safiyh
Hoffman, Adam
Zhang, Yian-Biao
Walla, Michael D.
Newman, Lee
Monchy, Sebastien
Issue Date: 2010
Citation: PLOS GENETICS, 6(5)
Abstract: Enterobacter sp. 638 is an endophytic plant growth promoting gamma-proteobacterium that was isolated from the stem of poplar (Populus trichocarpa x deltoides cv. H11-11), a potentially important biofuel feed stock plant. The Enterobacter sp. 638 genome sequence reveals the presence of a 4,518,712 bp chromosome and a 157,749 bp plasmid (pENT638-1). Genome annotation and comparative genomics allowed the identification of an extended set of genes specific to the plant niche adaptation of this bacterium. This includes genes that code for putative proteins involved in survival in the rhizosphere (to cope with oxidative stress or uptake of nutrients released by plant roots), root adhesion (pili, adhesion, hemagglutinin, cellulose biosynthesis), colonization/establishment inside the plant (chemiotaxis, flagella, cellobiose phosphorylase), plant protection against fungal and bacterial infections (siderophore production and synthesis of the antimicrobial compounds 4-hydroxybenzoate and 2-phenylethanol), and improved poplar growth and development through the production of the phytohormones indole acetic acid, acetoin, and 2,3-butanediol. Metabolite analysis confirmed by quantitative RT-PCR showed that, the production of acetoin and 2,3-butanediol is induced by the presence of sucrose in the growth medium. Interestingly, both the genetic determinants required for sucrose metabolism and the synthesis of acetoin and 2,3-butanediol are clustered on a genomic island. These findings point to a close interaction between Enterobacter sp. 638 and its poplar host, where the availability of sucrose, a major plant sugar, affects the synthesis of plant growth promoting phytohormones by the endophytic bacterium. The availability of the genome sequence, combined with metabolome and transcriptome analysis, will provide a better understanding of the synergistic interactions between poplar and its growth promoting endophyte Enterobacter sp. 638. This information can be further exploited to improve establishment and sustainable production of poplar as an energy feedstock on marginal, non-agricultural soils using endophytic bacteria as growth promoting agents.
Notes: [Taghavi, Safiyh; van der Lelie, Daniel; Hoffman, Adam; Zhang, Yian-Biao; Newman, Lee; Monchy, Sebastien] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. [Walla, Michael D.] Univ S Carolina, Dept Chem & Biochem, Mass Spectrometer Ctr, Columbia, SC 29208 USA. [Vangronsveld, Jaco] Univ Hasselt, Dept Environm Biol, Diepenbeek, Belgium. vdlelied@bnl.gov
URI: http://hdl.handle.net/1942/10979
DOI: 10.1371/journal.pgen.1000943
ISI #: 000278557300013
ISSN: 1553-7390
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
published version2.05 MBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.