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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10754

Title: Differential expression of equine muscle biopsy proteins during normal training and intensified training in young standardbred horses using proteomics technology
Authors: Bouwman, Freek G.
van Ginneken, Mireille M. E.
NOBEN, Jean-Paul
de Graaf-Roelfsema, Ellen
Wijnberg, Inge D.
van der Kolk, Johannes H.
Mariman, Edwin C. M.
van Breda, Eric
Issue Date: 2010
Abstract: The major aim of the present study was to investigate the proteome of standardbred horses at different stages of training and intensified training. We searched for biomarkers using small skeletal muscle biopsies of live animals. 2D gel electrophoresis and mass spectrometry were successfully applied to investigate training-induced differential expression of equine muscle biopsy proteins. Despite the poor resolution of the equine genome and proteome, we were able to identify the proteins of 20 differential spots representing 16 different proteins. Evaluation of those proteins complies with adaptation of the skeletal muscle after normal training involving structural changes towards a higher oxidative capacity, an increased capacity to take up long-chain fatty acids, and to store energy in the form of glycogen. Intensified training leads to additional changed spots. Alpha-l-antitrypsin was found increased after intensified training but not after normal training. This protein may thus be considered as a marker for overtraining in horses and also linked to overtraining in human athletes. (C) 2009 Elsevier Inc. All rights reserved.
Notes: [Bouwman, Freek G.; Mariman, Edwin C. M.] Maastricht Univ, NUTRIM, Dept Human Biol, Sch Nutr Toxicol & Metab,Med Ctr, NL-6200 MD Maastricht, Netherlands. [van Ginneken, Mireille M. E.; de Graaf-Roelfsema, Ellen; Wijnberg, Inge D.; van der Kolk, Johannes H.] Univ Utrecht, Med Sect, Dept Equine Sci, NL-3584 CM Utrecht, Netherlands. [Noben, Jean-Paul; Royackers, Erik] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul; Royackers, Erik] Transnat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [van Breda, Eric] Maastricht Univ, NUTRIM, Dept Human Movement Sci, Sch Nutr Toxicol & Metab,Med Ctr, NL-6200 MD Maastricht, Netherlands. e.mariman@hb.unimaas.nl
URI: http://hdl.handle.net/1942/10754
DOI: 10.1016/j.cbd.2009.11.001
ISI #: 000275070000006
ISSN: 1744-117X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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