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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10709

Title: Functional redundancy of genes for sulphate activation enzymes in Rhizobium sp. BR816.
Authors: Laeremans, T
Coolsaet, N
Verreth, C
Snoeck, C
Vanderleyden, J
Martinez-Romero, E
Issue Date: 1997
Publisher: Reading, England
Citation: MICROBIOLOGY, 143(12). p. 3933-3942
Abstract: The broad-host-range, heat-tolerant Rhizobium strain BR816 produces sulphated Nod metabolites. Two ORFs highly homologous to the Sinorhizobium meliloti nodPQ genes were isolated and sequenced. It was found that Rhizobium sp. BR816 contained two copies of these genes; one copy was localized on the symbiotic plasmid, the other on the megaplasmid. Both nodP genes were interrupted by insertion of antibiotic resistance cassettes, thus constructing a double nodP1P2 mutant strain. However, no detectable differences in Nod factor TLC profile from this mutant were observed as compared to the wild-type strain. Additionally, plant inoculation experiments did not reveal differences between the mutant strain and the wild-type. It is proposed that a third, functionally homologous locus complements mutations in the Nod factor sulphation genes. Southern blot analysis suggested that this locus contains genes necessary for the sulphation of amino acids.
Notes: Departamento de Genetica Molecular, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico.
URI: http://hdl.handle.net/1942/10709
ISSN: 1350-0872
Type: Journal Contribution
Appears in Collections: Research publications

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