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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10446

Title: Breakdown of thermodynamic equilibrium for DNA hybridization in microarrays
Authors: Hooyberghs, Jef
Baiesi, Marco
Ferrantini, A.
Carlon, E.
Issue Date: 2010
Citation: PHYSICAL REVIEW E, 81(1)
Abstract: Test experiments of hybridization in DNA microarrays show systematic deviations from the equilibrium isotherms. We argue that these deviations are due to the presence of a partially hybridized long-lived state, which we include in a kinetic model. Experiments confirm the model predictions for the intensity vs free-energy behavior. The existence of slow relaxation phenomena has important consequences for the specificity of microarrays as devices for the detection of a target sequence from a complex mixture of nucleic acids.
Notes: [Hooyberghs, J.] Vlaamse Instelling Technol Onderzoek, Flemish Inst Technol Res, B-2400 Mol, Belgium. [Hooyberghs, J.; Baiesi, M.; Ferrantini, A.; Carlon, E.] Katholieke Univ Leuven, Inst Theoret Phys, B-3001 Heverlee, Belgium. [Hooyberghs, J.] Hasselt Univ, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/10446
DOI: 10.1103/PhysRevE.81.012901
ISI #: 000274003300099
ISSN: 1539-3755
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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