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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10434

Title: Biological modification of carbon nanowalls with DNA strands and hybridization experiments with complementary and mismatched DNA
Authors: VANSWEEVELT, Rob
Malesevic, A.
VAN GOMPEL, Matthias
Vanhulsel, A.
WENMACKERS, Sylvia
D'HAEN, Jan
VERMEEREN, Veronique
AMELOOT, Marcel
MICHIELS, Luc
Van Haesendonck, C.
WAGNER, Patrick
Issue Date: 2010
Publisher: ELSEVIER SCIENCE BV
Citation: CHEMICAL PHYSICS LETTERS, 485(1-3). p. 196-201
Abstract: Carbon nanowalls (CNWs) are functionalized with short DNA strands. CNWs are a network of upstanding carbon flakes consisting of 4-6 graphene layers packed together. The biological modi. cation of these structures is carried out via the photochemical attachment of an unsaturated fatty acid linker molecule, followed by an EDC-mediated reaction to bind amino-modified DNA strands to the linker. Hybridization experiments are done using complementary and mismatch DNA strands. Fluorescence microscopy shows a clear difference between EDC-negative and EDC-positive samples, supporting the covalent nature of the DNA attachment. Furthermore, we demonstrate that the hybridization is highly selective and reproducible. (C) 2009 Elsevier B. V. All rights reserved.
Notes: [Vansweevelt, R.; Van Gompel, M.; Wenmackers, S.; D'Haen, J.; Wagner, P.] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Malesevic, A.; Vanhulsel, A.] Flemish Inst Technol Res, VITO Mat, B-2400 Mol, Belgium. [Malesevic, A.; Van Haesendonck, C.] Katholieke Univ Leuven, Lab Solid State Phys & Magnetism, B-3001 Louvain, Belgium. [Vermeeren, V.; Ameloot, M.; Michiels, L.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Wagner, P.] IMEC, Div IMOMEC, B-3590 Diepenbeek, Belgium. rob.vansweevelt@uhasselt.be
URI: http://hdl.handle.net/1942/10434
DOI: 10.1016/j.cplett.2009.12.040
ISI #: 000273231900040
ISSN: 0009-2614
Category: A1
Type: Journal Contribution
Validation: ecoom, 2011
Appears in Collections: Research publications

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