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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10410

Title: Survey of Pseudomonas aeruginosa and its phages: de novo peptide sequencing as a novel tool to assess the diversity of worldwide collected viruses.
Authors: Ceyssens, Pieter-Jan
NOBEN, Jean-Paul
Ackermann, Hans-W.
Verhaegen, Jan
De Vos, Daniel
Pirnay, Jean-Paul
Merabishvili, Maia
Vaneechoutte, Mario
Chibeu, Andrew
Volckaert, Guido
Lavigne, Rob
Issue Date: 2009
Citation: Environmental microbiology, 11(5). p. 1303-1313
Abstract: A collection of 15 newly isolated (bacterio)phages infecting the opportunistic pathogen Pseudomonas aeruginosa was established to investigate their global diversity and potential in phage therapy. These phages were sampled in 14 different countries traversing four continents, from both natural environments and hospital sewage. They all display unique DNA and protein profiles and cluster morphologically into six groups within the three major families of the Caudovirales. Extensive host range studies on a library of 122 AFLP-genotyped clinical P. aeruginosa strains (of which 49 were newly isolated at the University Hospital of Leuven, Belgium) showed that the phages lysed 87% of the strains. Infection analysis of outer membrane mutants identified 10 phages as type IV pili-dependent. More detailed information about the evolutionary relatedness of the phages was gathered by de novo peptide sequencing of major virion proteins using tandem Matrix-Assisted Laser Desorption/Ionization Time of Flight technology. Applying this technique for the first time to viruses, seven groups of closely related phages were identified without the need of prior knowledge of genome content and/or electron microscopic imaging. This study demonstrates both the epidemic population structure of P. aeruginosa and the global spread of P. aeruginosa phage species, and points at the resistance of two clinically predominant, widespread P. aeruginosa strains against phage attack.
Notes: Reprint Address: Lavigne, R (reprint author), Katholieke Univ Leuven, Div Gene Technol, Kasteelpk Arenberg 21, Louvain, Belgium Addresses: 1. Katholieke Univ Leuven, Div Gene Technol, Louvain, Belgium 2. Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium 3. Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium 4. Univ Laval, Fac Med, Dept Med Biol, Felix Herelle Reference Ctr Bacterial Viruses, Quebec City, PQ G1K 7P4 Canada 5. Katholieke Univ Leuven, Div Expt Lab Med, Louvain, Belgium 6. Hosp Ctr Base Queen Astrid, Lab Mol & Cellular Technol, Burn Unit, B-1120 Brussels, Belgium 7. Ghent Univ Hosp, Dept Clin Chem Microbiol & Immunol, Lab Bacteriol Res, B-9000 Ghent, Belgium 8. EIBMV, Tbilisi, Rep of Georgia E-mail Addresses: rob.lavigne@biw.kuleuven.be
URI: http://hdl.handle.net/1942/10410
DOI: 10.1111/j.1462-2920.2008.01862.x
ISI #: 000265481600022
ISSN: 1462-2912
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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