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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10339

Title: Analysis of Mitochondrial pH and Ion Concentrations
Authors: VAN DE VEN, Martin
Balut, Corina
Baron, Szilvia
SMETS, Ilse
STEELS, Paul
AMELOOT, Marcel
Issue Date: 2010
Publisher: Humana Press
Citation: Papovski, DB (Ed.) Life Cell Imaging, p. 275-309.
Series/Report: Methods in Molecular Biology, 591
Abstract: Detailed practical information is provided with emphasis on mapping cytosolic and mitochondrial pH, mitochondrial Na+, and briefly also aspects related to mitochondrial Ca2+ measurements in living cells, as grown on (un)coated glass coverslips. This chapter lists (laser scanning confocal) microscope instrumentation and setup requirements for proper imaging conditions, cell holders, and an easy-to-use incubator stage. For the daily routine of preparing buffer and calibration solutions, extensive annotated protocols are provided. In addition, detailed measurement and image analysis protocols are given to routinely obtain optimum results with confidence, while avoiding a number of typical pitfalls.
URI: http://hdl.handle.net/1942/10339
Link to publication: 10.1007/978-1-60761-404-3
DOI: 10.1007/978-1-60761-404-3
ISBN: 978-1-60761-405-0
Category: B2
Type: Book Section
Appears in Collections: Research publications

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