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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10338

Title: Practical Time-Resolved Fluorescence Spectroscopy: Avoiding Artifacts and Using Lifetime Standards
Authors: Boens, N
Valeur, B
Issue Date: 2008
Publisher: Springer Berlin Heidelberg
Citation: Resch_Genger, U (Ed.) Standardization and Quality Assurance in Fluorescence Measurements I, p. 215-232.
Series/Report: Springer Series on Fluorescence, 5
Abstract: In this chapter we describe how artifacts can be avoided in the two most commonly used time-resolved fluorometries, namely the single-photon timing and the multifrequency phase-modulation techniques. The most frequently encountered artifacts (inner filter effect, autofluorescence, polarization effects, color effect, photobleaching, deoxygenation, pulse pile-up, and linearity of the time response in the time-to-amplitude converter) are described in detail and remedies are presented to avoid these pitfalls. An extensive list of fluorescence lifetime standards is presented, which allows the spectroscopist to calibrate and test time-resolved instruments for systematic errors.
URI: http://hdl.handle.net/1942/10338
DOI: 10.1007/4243_2008_044
ISBN: 978-3-540-75206-6
Category: B2
Type: Book Section
Appears in Collections: Research publications

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