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Title: Human Papillomavirus 16 Load and E2/E6 Ratio in HPV16-Positive Women: Biomarkers for Cervical Intraepithelial Neoplasia >= 2 in a Liquid-Based Cytology Setting?
Authors: Boulet, Gaelle A. V.
Benoy, Ina H.
Depuydt, Christophe E.
Horvath, Caroline A. J.
HENS, Niel
Vereecken, Annie J.
Bogers, Johannes J.
Issue Date: 2009
Abstract: This retrospective case-control study assessed human papillomavirus 16 (HPV16) viral load and E2/E6 ratio as risk markers for cervical intraepithelial neoplasia WIN) >= 2 lesions in HPV16-positive women in a routine liquid-based cytology setting. Triplex quantitative PCR for HPV16 E6, E2, and beta-globin was done to determine the HPV16 load and the E2/E6 ratio, as a surrogate marker for integration, for women with a negative histologic endpoint (200 controls: 83 normal histology and 117 CIN1) and women with a >= CIN2 endpoint (180 cases: 41 CIN2, 122 CIN3, and 17 invasive carcinoma). Our analysis showed a significantly higher HPV16 load in the case group, which was completely attributable to the high viral load of samples with invasive carcinoma as histologic endpoint. There was no significant difference in viral load between the other histologic groups. The E2/E6 ratio proved to be lower for the cases. However, the E2/E6 ratio indicated the presence of HPV integration in a considerable amount of control samples (44.3%), which suggests that HPV integration occurs early in the development of cancer and undermines the clinical value of viral integration. Overall, the intrinsic heterogeneous nature of the cervical cytology samples caused a substantial overlap of the HPV16 load and the E2/E6 ratio between controls and cases, which precludes the determination of cutoff values for risk prediction and hampers the clinical applicability in a cervical screening setting. (Cancer Epidemiol Biomarkers Prev 2009;18(11):2992-9)
Notes: [Benoy, Ina H.; Depuydt, Christophe E.; Vereecken, Annie J.; Bogers, Johannes J.] Lab Clin Pathol Labo Lokeren Campus RIATOL, B-2000 Antwerp, Belgium. [Boulet, Gaelle A. V.; Horvath, Caroline A. J.; Bogers, Johannes J.] Univ Antwerp, Cell Biol & Histol Lab, Appl Mol Biol Res Grp, B-2020 Antwerp, Belgium. [Aerts, Marc; Hens, Niel] Univ Hasselt, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. [Aerts, Marc; Hens, Niel] Katholieke Univ Leuven, Leuven, Belgium.
URI: http://hdl.handle.net/1942/10297
DOI: 10.1158/1055-9965.EPI-09-0025
ISI #: 000271562600028
ISSN: 1055-9965
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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