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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10282

Title: LIVER X RECEPTORS IN CHOLESTEROL HOMEOSTASIS IN PRIMARY RAT OLIGODENDROCYTES
Authors: NELISSEN, Katherine
HENDRIKS, Jerome
Smets, I
SMEETS, Karen
Mulder, M.
AMELOOT, Marcel
Issue Date: 2009
Publisher: WILEY-LISS
Citation: GLIA, 57(13). p. S51-S51
Abstract: Cholesterol synthesis and transport in oligodendrocytes are essential for optimal myelination and remyelination in pathological conditions such as multiple sclerosis.However, little is known about cholesterol homeostasis in the myelin-forming oligondendrocytes. Liver X receptors (LXR's) are nuclear oxysterol receptors that regulate genes involved in cholesterol homeostasis and may therefore play an important role in de- and remyelination. We investigated whether LXRs regulate cholesterol homeostasis in oligodendrocytes. mRNA expression of genes encoding LXR-α and LXR-β and their target genes (ABCA1, ABCG1, ABCG4, apoE, and LDLR) was detected in oligodendrocytes derived from both neonatal and adult rats using quantitative real-time PCR. The expression of LXR-β and several target genes was increased during oligodendrocyte differentiation. We further demonstrated that treatment of primary neonatal rat oligodendrocytes with the synthetic LXR agonist T0901317 induced the expression of several established LXR target genes, including ABCA1, ABCG1, apoE, and LDLR. Treatment of oligodendrocytes with T0901317 resulted in an enhanced cholesterol efflux in the presence of apolipoprotein A-I or high-density lipoprotein particles. These data show that LXRs are involved in regulating cholesterol homeostasis in oligodendrocytes.
Notes: [Nelissen, K.; Hendriks, J.; Smeets, K.; Ameloot, M.] Hasselt Univ, Diepenbeek, Belgium. [Smets, I] PHL Univ Coll, Diepenbeek, Belgium. [Mulder, M.] Rotterdam Univ, Rotterdam, Netherlands.
URI: http://hdl.handle.net/1942/10282
ISI #: 000270075500211
ISSN: 0894-1491
Category: M
Type: Journal Contribution
Appears in Collections: Research publications

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