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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/10276

Title: Comparative analysis of the widespread and conserved PB1-like viruses infecting Pseudomonas aeruginosa
Authors: Ceyssens, Pieter-Jan
Miroshnikov, Konstantin A.
Mattheus, Wesley
Krylov, Victor
NOBEN, Jean-Paul
Vanderschraeghe, Simon
Sykilinda, Nina
Kropinski, Andrew M.
Volckaert, Guido
Mesyanzhinov, Vadim V.
Lavigne, Rob
Issue Date: 2009
Citation: ENVIRONMENTAL MICROBIOLOGY, 11(11). p. 2874-2883
Abstract: We examined the genetic diversity of lytic Pseudomonas aeruginosa bacteriophage PB1 and four closely related phages (LBL3, LMA2, 14-1 and SN) isolated throughout Europe. They all encapsulate linear, non-permuted genomes between 64 427 and 66 530 bp within a solid, acid-resistant isometric capsid (diameter: 74 nm) and carry non-flexible, contractile tails of approximately 140 nm. The genomes are organized into at least seven transcriptional blocks, alternating on both strands, and encode between 88 (LBL3) and 95 (LMA2) proteins. Their virion particles are composed of at least 22 different proteins, which were identified using mass spectrometry. Posttranslational modifications were suggested for two proteins, and a frameshift hotspot was identified within ORF42, encoding a structural protein. Despite large temporal and spatial separations between phage isolations, very high sequence similarity and limited horizontal gene transfer were found between the individual viruses. These PB1-like viruses constitute a new genus of environmentally very widespread phages within the Myoviridae.
Notes: [Ceyssens, Pieter-Jan; Mattheus, Wesley; Vanderschraeghe, Simon; Volckaert, Guido; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, Louvain, Belgium. [Miroshnikov, Konstantin; Sykilinda, Nina; Mesyanzhinov, Vadim] Inst Bioorgan Chem, Moscow 117997, Russia. [Krylov, Victor] State Inst Genet Ind Microorganisms, Moscow 113545, Russia. [Robben, Johan; Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Robben, Johan; Noben, Jean-Paul] Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [Kropinski, Andrew M.] Lab Foodborne Zoonoses, Guelph, ON N1G 3W4, Canada. [Kropinski, Andrew M.] Univ Guelph, Guelph, ON N1G 2W1, Canada.
URI: http://hdl.handle.net/1942/10276
DOI: 10.1111/j.1462-2920.2009.02030.x
ISI #: 000271244800010
ISSN: 1462-2912
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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