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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1002

Title: Profiling the autoantibody repertoire by serological antigen selection
Authors: SOMERS, Veerle
Hupperts, R.
Issue Date: 2005
Publisher: Elsevier
Citation: JOURNAL OF AUTOIMMUNITY, 25(3). p. 223-228
Abstract: The identification of disease related autoantigens targeted by pathogenic T- and B-cell responses is crucial for the development of improved therapies for autoimmune diseases. To identify immunogenic targets recognized by the humoral immune response, we have recently applied a novel and powerful molecular approach, named ‘serological antigen selection’ (SAS). This method involves the display of a cDNA expression library on filamentous phage and subsequent selection on patient immunoglobulin G (IgG). In the present study, we have cloned a cDNA repertoire from a multiple sclerosis (MS) patient in pVI phage display vectors and performed selections on pooled MS cerebrospinal fluid (CSF) samples immobilized with anti-human IgG. To further streamline this procedure, we report an optimized SAS procedure in which we have successfully established methods for enrichment of MS-specific candidate antigens. In conclusion, the broad applicability of the SAS method makes it a highly promising method for investigating the autoimmune repertoire.
URI: http://hdl.handle.net/1942/1002
DOI: 10.1016/j.jaut.2005.09.023
ISI #: 000233891200008
ISSN: 0896-8411
Category: A1
Type: Journal Contribution
Validation: ecoom, 2006
Appears in Collections: Research publications

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